对Bacillus anthracis的毒性菌株进行比较基因组分析,这些菌株在动物中引起炭病爆发
Awadhesh Prajapati1,2, Sripada Sairam1, Suresh Bindu1
1ICAR-National Institute of Veterinary Epidemiology and Disease Informatics (NIVEDI), Post Box No. 6450, Yelahanka, Bengaluru 560 119, Karnataka, India.
Genome
|November 19, 2025
概括
这项研究分析了来自印度牲畜的Bacillus anthracis基因组,揭示了牛羊中循环的多样化子系. 这些发现强调了A.Br.003亚系在动物炭病例中的流行率.
科学领域:
- 基因组学就是基因组学.
- 微生物学 微生物学
- 兽医科学 兽医科学 兽医科学
背景情况:
- 炭是一种致命的动物传染病,由炭杆菌 (Bacillus anthracis) 引起,对牲畜和人类健康构成重大威胁.
- 了解Bacillus anthracis菌株的遗传多样性对于有效的疾病控制和预防策略至关重要.
研究的目的:
- 对来自牛和绵羊的两种印度毒性百菌 (Bacillus anthracis) 菌株 (NIVEDIAX3和NIVEDIAX61) 进行全基因组比较分析.
- 为了将这些菌株与NCBI数据库中的其他55个可用的Bacillus anthracis基因组进行比较.
- 调查印度Bacillus anthracis的遗传关系,遗传构成和子系分布.
主要方法:
- 全基因组测序和比较基因组分析.
- 使用平均核酸标识 (ANI) 的基因组学分析.
- 多位元序列类型 (MLST) 和正规单核酸多态 (canSNP) 分析.
主要成果:
- 两种印度菌株NIVEDIAX3和NIVEDIAX61与艾姆斯祖先菌株在Cluster II.下聚集在一起.
- 在MLST分析中,Bacillus cereus ST1,Bacillus anthracis核心基因组MLST ST284和Bacillus anthracis等离子体ST12的菌株被分配.
- 两种印度菌株都被归类为A.Br.003 (A.Br.Aust94亚系),这被发现是研究中动物分离物中最常见的canSNP.
- 对57个Bacillus anthracis基因组的分析确定了NIVEDIAX菌株和Ames Ancestor之间共享的正统和单拷贝基因集群.
结论:
- 这项研究确定了印度南部和东部的牲畜中多样化的Bacillus anthracis亚系的循环.
- A.Br.003亚系是印度动物炭病例中显著且普遍存在的基因型.
- 基因组洞察力有助于理解炭病流行病学,并为公共卫生干预提供信息.
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