相关实验视频

Updated: Jan 11, 2026

A Three-dimensional Model of Spheroids to Study Colon Cancer Stem Cells

A Three-dimensional Model of Spheroids to Study Colon Cancer Stem Cells

Published on: January 22, 2021

层状双氧化物加载的miR-654-3p针对POU2F1介导的代谢重编程来对抗结直肠癌

Jiwen Shi¹, Chengle Zhu², Shaopeng Xu³

¹Anhui Provincial Key Laboratory of Tumor Evolution and Intelligent Diagnosis and Treatment, Bengbu Medical University, 2600 Donghai Avenue, Bengbu, Anhui 233030, China; Anhui Engineering Research Center for Neural Regeneration Technology and Medical New Materials,Anhui 233030, China.

Bioorganic chemistry

|November 19, 2025

概括

这项研究揭示了结直肠癌 (CRC) 中的低miR-654-3p水平通过影响代谢重编程和上皮-介质细胞过渡 (EMT) 来促进瘤生长. miR-654-3p的纳米粒子输送显示了CRC治疗的潜力.

科学领域:

在瘤学瘤学.
分子生物学分子生物学
纳米技术纳米技术

背景情况:

结肠直肠癌 (CRC) 是一种严重的健康威胁,由代谢重编程和瘤微环境 (TME) 改变驱动.
微RNAs (miRNAs) 是TME中的关键调节者,影响癌症的进展.

研究的目的:

研究miR-654-3p在结肠直肠癌中的作用.
阐明miR-654-3p影响CRC进展的分子机制,包括代谢重编程,自和上皮-介质细胞过渡 (EMT).
评估使用分层双氧化物 (LDH) 纳米颗粒为CRC治疗提供miR-654-3p的治疗潜力.

主要方法:

在CRC患者的血外体上进行了miRNA测序 (miRNA-seq),以确定差异表达的miRNAs.
为了分析miR-654-3p的分子机制,进行了体内和体外实验.
层层的双氧化物 (LDH) 纳米粒子被合成并加载了miR-654-3p,以创建用于治疗评估的LDH@miR-654-3p纳米复合材料.

主要成果:

在CRC患者的血外体中检测到miR-654-3p的低表达.
发现miR-654-3p针对POU2F1,抑制CRC中的代谢重编程,自和上皮-介质细胞转换 (EMT).
在CRC模型中,LDH@miR-654-3p纳米复合材料显示出显著的抗瘤功效.

结论:

miR-654-3p通过向POU2F1并调节关键癌症特征,作为结直肠癌中的瘤抑制剂.
开发的LDH@miR-654-3p纳米治疗系统显示出在CRC治疗中临床转换的前景.
这些发现为针对结直肠癌的新型纳米治疗策略提供了机制基础.

关键词:

结肠直肠癌是一种癌症.这是一种层状双氧化物.代谢重编程是一种代谢重编程.在 miR-654-3p 中使用.

更多相关视频

Development and Maintenance of a Preclinical Patient Derived Tumor Xenograft Model for the Investigation of Novel Anti-Cancer Therapies

Development and Maintenance of a Preclinical Patient Derived Tumor Xenograft Model for the Investigation of Novel Anti-Cancer Therapies

Published on: September 30, 2016

A Multiplexed Luciferase-based Screening Platform for Interrogating Cancer-associated Signal Transduction in Cultured Cells

A Multiplexed Luciferase-based Screening Platform for Interrogating Cancer-associated Signal Transduction in Cultured Cells

Published on: July 3, 2013

相关概念视频

Targeted Cancer Therapies

Targeted Cancer Therapies

The targeted cancer therapies, also known as “molecular targeted therapies,” take advantage of the molecular and genetic differences between the cancer cells and the normal cells. It needs a thorough understanding of the cancer cells to develop drugs that can target specific molecular aspects that drive the growth, progression, and spread of cancer cells without affecting the growth and survival of other normal cells in the body.
There are several types of targeted therapies against...

Abnormal Proliferation

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...