用Corynebacterium glutamicum进行维生素B12的代谢工程 - - 独立生产3-基酸
Cheon Woo Moon1, Mohammad Rifqi Ghiffary2, Cindy Pricilia Surya Prabowo1
1Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering (BK21 Four Program), KAIST Institute for BioCentury, KAIST, Daejeon, 34141, Republic of Korea; Systems Metabolic Engineering and Systems Healthcare Cross-Generation Collaborative Laboratory, KAIST, Daejeon, 34141, Republic of Korea.
Metabolic engineering
|November 19, 2025
概括
研究人员开发了一种不依赖于维生素B12的途径,用于微生物生产3-基酸 (3-HP). 这种可持续的方法可以从葡萄糖中实现高水平的3HP发酵,克服了以前工业应用的生物合成路径的局限性.
科学领域:
- 生物技术是生物技术.
- 合成生物学 合成生物学
- 代谢工程是代谢工程.
背景情况:
- 3-基酸 (3-HP) 是合成有价值化学品和可生物降解聚合物的关键平台化学品.
- 目前的工业3HP生产依赖于不可持续的化学合成.
- 微生物生物合成提供了一个可持续的替代方案,但现有的途径往往取决于昂贵的维生素B12辅因子,限制了可扩展性.
研究的目的:
- 在Corynebacterium glutamicum中开发一种维生素B12独立的生物合成途径,用于高水平的3-HP生产.
- 从葡萄糖中设计一种微生物菌株,以实现高效且可扩展的3-HP发酵性生产.
主要方法:
- 引入了一种新的维生素B12独立途径,该途径来自β-氨酸,用于3HP合成.
- 通过提高前体可用性和辅因子平衡,优化了β-氨酸过度产生的菌株 (BAL10) 的途径.
- 加强了β-氨酸生物合成途径,并识别/调整了一个3HP输送器.
主要成果:
- 从葡萄糖中获得126.3g/L的3-HP的高水平发酵生产.
- 获得0.36g/g葡萄糖的高产量和1.75g/L/h的整体生产率.
- 证明了维生素B12独立路线的可行性,用于工业3HP生产.
结论:
- 开发的维生素B12独立途径是可持续和可扩展的微生物生产3-HP的可行策略.
- 这一进步克服了当前3HP生物合成的关键局限性,为工业应用铺平了道路.
- 改造的Corynebacterium glutamicum菌株显示出具有成本效益和环保的3HP制造的巨大潜力.
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