在亚麻织物上固定热稳定的β-葡萄糖酶的特性
Karine Dyukova1, Ani Paloyan1, Lev Khoyetsyan1
1Scientific and Production Center "Armbiotechnology" National Academy of Science of Armenia, 0014 Yerevan, Armenia.
Journal of dairy science
|November 19, 2025
概括
研究人员开发了一种可重复使用的生物催化剂,使用热稳定的β-葡萄糖酶固定在亚麻布上,用于有效的乳糖水解. 这种环保的方法提高了酶的稳定性和可重复使用性,为乳制品副产品的价值化提供了可持续的解决方案.
科学领域:
- 生物技术是生物技术.
- 酵素工程是什么意思 酵素工程
- 绿色化学 绿色化学
背景情况:
- 来自极端环境的热稳定酶提供了工业潜力.
- 乳糖水解对于乳制品加工和副产品价值化至关重要.
- 开发可重复使用和环保的生物催化剂是生物技术的一个关键挑战.
研究的目的:
- 为了使热稳定的β-葡萄糖酶固定在亚麻布上.
- 为了评估乳糖水解的固定酶的性能和稳定性.
- 评估亚麻织物作为工业生物催化剂的载体的潜力.
主要方法:
- 通过直接共价结合和间隔臂策略在亚麻布上固定β-葡萄糖酶.
- 酶活性,pH值和温度的特性,以及自由和固定酶的稳定性.
- 评估生物催化剂的可重复使用性和在奶酪乳清处理中的应用.
主要成果:
- 间隔臂固定化策略 (LfEG-β-葡萄糖酶) 产生了优异的性能,保持了76.6%的活性.
- 固定化的酶显示出增强的热和储存稳定性,在4°C下1年后保持完全活性.
- 生物催化剂在15个重复使用周期后在奶酪乳清处理中保持了90%的活性,产生6.4g/L的葡萄糖.
结论:
- 可生物降解的亚麻布是热稳定酶的可行,低成本的载体.
- 开发的生物催化剂为乳糖水解提供了高效率和可重复使用性.
- 这项技术支持可持续的乳制品行业实践和副产品价值化.
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