表面修饰的奇托桑稳定纳米颗粒与带有差异电荷的硫配体,用于抗菌和抗生物膜应用
Navjot Kaur1, Selvakumar Thriambakeshwar1, Mrinmoy De1
1Department of Organic Chemistry, Indian Institute of Science, Bengaluru, India.
Chemistry, an Asian journal
|November 19, 2025
概括
纳米颗粒 (SeNPs) 的表面化学修饰增强了抗菌活性. 基托桑稳定型SeNPs (CS-SeNPs) 上的阴离子表面涂层显著提高了对黄金葡萄球菌和大肠杆菌 (包括生物膜) 的疗效,具有低哺乳动物细胞毒性.
科学领域:
- 纳米材料科学是一门学科.
- 表面化学 表面化学
- 抗微生物工程 抗微生物工程
背景情况:
- 表面化学决定了纳米材料与微生物的相互作用,影响了抗菌性质.
- 基托稳定纳米粒子 (CS-SeNPs) 是抗菌应用的一个有前途的平台.
- 二次表面涂料对CS-SeNPs生物活性的影响需要进一步研究.
研究的目的:
- 通过双层涂层策略,设计具有增强抗菌和抗菌膜功效的CS-SeNPs.
- 调查不同头组功能 (阴离子,阴离子,中性) 对CS-SeNPs抗菌性能的影响.
- 阐明强大的CS-SeNPs对细菌病原体的作用机制.
主要方法:
- 合成CS-SeNPs使用酸减少化与奇托稳定.
- 在CS-SeNPs的后功能化中,使用带有阴离子,阴离子或中性头组的硫化联体.
- 对抗黄金葡萄球菌和大肠杆菌的抗菌活性 (MIC/MBC) 和对成熟生物膜的抗菌膜疗效的评估.
- 通过产生活性氧物种 (ROS),膜脱极化和膜破裂试验来评估作用方式.
- 在哺乳动物细胞上进行初步生物相容性测试.
主要成果:
- 电离@CS-SeNPs对S. aureus和E. coli表现出显著的抗菌活性,MIC/MBC值分别为1/2微克/毫升和2.5/20微克/毫升.
- 阴性@CS-SeNPs和中性@CS-SeNPs在测试度范围内没有表现出抑制.
- 与未经修改的CS-SeNPs相比,Cationic@CS-SeNPs显示MIC对S. aureus的改善约为70倍.
- 通过使用Cationic@CS-SeNPs.观察到成熟的S. aureus和E. coli生物膜的有效抑制.
- 作用机制包括ROS生成,膜脱极化和膜破裂.
- 初步测试表明,Cationic@CS-SeNPs对哺乳动物细胞的细胞毒性较低.
结论:
- 聚合物覆盖的SeNPs的联体工程提供了一种模块化方法,以提高抗菌和抗菌膜的疗效.
- 阴离子表面功能化是开发强大的CS-SeNP对抗浮游生物和生物膜嵌入细菌的关键策略.
- 这些发现为开发具有更好的安全概况的先进抗微生物纳米材料提供了一条途径.
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