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Super-resolution Fluorescence Microscopy

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...

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Updated: Jan 10, 2026

Three-dimensional Super Resolution Microscopy of F-actin Filaments by Interferometric PhotoActivated Localization Microscopy iPALM

Three-dimensional Super Resolution Microscopy of F-actin Filaments by Interferometric PhotoActivated Localization Microscopy iPALM

Published on: December 1, 2016

开源的亚纳米稳定系统用于超分辨率光显微镜.

Florencia Edorna^1,2, Florencia D Choque^1,2, Giovanni Ferrari^1,3

¹Centro de Investigaciones en Bionanociencias (CIBION), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Ciudad Autónoma de Buenos Aires, Argentina.

Light, science & applications

|November 19, 2025

概括

此摘要是机器生成的。

我们开发了一种用于超分辨率显微镜的活性稳定系统,在数小时内达到亚纳米精度. 这个系统增强了像MINFLUX和RASTMIN这样可视化单个分子的技术.

更多相关视频

Test Samples for Optimizing STORM Super-Resolution Microscopy

Test Samples for Optimizing STORM Super-Resolution Microscopy

Published on: September 6, 2013

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

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Published on: December 9, 2013

相关实验视频

Last Updated: Jan 10, 2026

Three-dimensional Super Resolution Microscopy of F-actin Filaments by Interferometric PhotoActivated Localization Microscopy iPALM

Three-dimensional Super Resolution Microscopy of F-actin Filaments by Interferometric PhotoActivated Localization Microscopy iPALM

Published on: December 1, 2016

Test Samples for Optimizing STORM Super-Resolution Microscopy

Test Samples for Optimizing STORM Super-Resolution Microscopy

Published on: September 6, 2013

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

Published on: December 9, 2013

科学领域:

光学显微镜是一种光学显微镜.
纳米技术纳米技术
生物物理学的生物物理.

背景情况:

超分辨率光显微镜实现1-10纳米分辨率可可视化单个分子.
高精度需要严格的样本漂移控制,特别是对于MINFLUX和RASTMIN等技术.
积极的漂移校正对于显微镜中的纳米尺度分辨率至关重要.

研究的目的:

为超分辨率显微镜提供一个活性稳定系统.
为了长时间实现亚纳米精度稳定.
为增强显微镜性能提供可适应和开源解决方案.

主要方法:

为主动稳定模块开发一个简单的光学设计.
集成开源控制软件与用户友好的图形界面.
使用p-MINFLUX和RASTMIN测量在各种实验设置中的演示.

主要成果:

稳定系统可以在数小时内提供亚纳米精度.
该系统可作为各种光显微镜的模块进行调整.
测量结果达到了理论上的克拉梅尔 - 拉奥界限,在DNA原始化中解决了~10nm距离.

结论:

活动稳定系统显著提高了超分辨率显微镜的精度.
开源性质和适应性促进了广泛采用.
该系统能够在生物环境中对纳米结构进行强大的可视化.