相关实验视频

Updated: Jan 10, 2026

Resolving Affinity Purified Protein Complexes by Blue Native PAGE and Protein Correlation Profiling

Resolving Affinity Purified Protein Complexes by Blue Native PAGE and Protein Correlation Profiling

Published on: April 1, 2017

组织核酸结合蛋白质组的大规模破译通过亲和性染色学与数据独立的基于获取的蛋白质组学相结合

Huiyu Wang^1,2, Jianzheng Zhu¹, Suntao Li¹

¹State Key Laboratory of Microbial Metabolism, Joint International Research Laboratory of Metabolic & Developmental Sciences, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China.

Nucleic acids research

|November 20, 2025

概括

一种新的NABP-DIA方法增强了核酸结合蛋白质组 (NABPome) 在炎症性肠病中的蛋白质组分析. 表观遗传调节,特别是H3K27me3抑制,通过减少炎症因素,显示出治疗大肠炎的前途.

科学领域:

蛋白质组学是指蛋白质组学.
表观遗传学在表观遗传学中,表观遗传学是指表观遗传学.
免疫学免疫学免疫学

背景情况:

炎症性肠病 (IBD) 的发病包括核酸结合蛋白质 (NABPome) 的转录和表观遗传调节者.
由于当前蛋白质组学策略的局限性,NABPome的全面分析受到阻碍.

研究的目的:

开发一种先进的蛋白质组学方法来深入分析NABPome的组成和动态.
在大肠炎模型中研究NABPome的变化,并探索潜在的治疗点.

主要方法:

开发NABP-DIA方法,将NABPome捕获与数据独立获取 (DIA) 蛋白质组合.
利用实验生成的光谱库来增强NABP的识别和量化.
将NABP-DIA应用于小鼠结肠炎模型,分析脏和胸腺组织.

主要成果:

使用NABP-DIA实现了NABP识别的2.1倍增长,使用NABP-DIA提高了准确性和特异性.
在大肠炎小鼠的脏中鉴定了118个差异表达的NABP,在胆髓中25个.
在结肠炎组中观察到脏蛋白质升高与基因素甲基化相关.

结论:

NABP-DIA是全面NABPome分析的一个强大的工具.
表观遗传失调,特别是基因组甲基化,与大肠炎有关.
抑制H3K27me3基因组甲基化减少了炎症因子的释放,这表明大肠炎的潜在治疗策略.

更多相关视频

Navigating the Mass Spectrometry-Based Proteomic Data Using Free Computational Tools

Navigating the Mass Spectrometry-Based Proteomic Data Using Free Computational Tools

Published on: August 19, 2025

Mapping Dysfunctional Protein-Protein Interactions in Disease

Mapping Dysfunctional Protein-Protein Interactions in Disease

Published on: October 24, 2025

相关概念视频

Peptide Identification Using Tandem Mass Spectrometry

Peptide Identification Using Tandem Mass Spectrometry

Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...

Affinity Chromatography

Affinity Chromatography

Affinity chromatography is a powerful technique extensively utilized for separating and purifying specific biomolecules from complex mixtures. It capitalizes on the highly selective binding between an analyte and its counterpart, such as antibody-antigen interactions. The counterpart is immobilized on the stationary phase, forming an affinity column. The stationary phase typically consists of solid support, such as agarose or porous glass beads, immobilizing the affinity ligand. The mobile...