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ASTRA:人类细胞系中压力诱导的转录活动的全面资源
Margaritis Tsifintaris1, Dimitris Grigoriadis1,2, Michail Sitmalidis1
1Department of Molecular Biology and Genetics, Democritus University of Thrace, Alexandroupolis 68100, Greece.
Nucleic acids research
|November 20, 2025
概括
压力反应活动图谱 (ASTRA) 数据库提供了各种压力下人类细胞系的标准化转录基因数据. 本资源有助于发现应激反应基因和RNA机制,用于细胞平衡和疾病研究.
科学领域:
- 基因组学就是基因组学.
- 系统生物学 系统生物学
- 翻译研究是翻译研究.
背景情况:
- 了解细胞对压力的反应对于破译疾病机制和维持平衡至关重要.
- 在各种压力条件下的转录数据通常是碎片化的,缺乏标准化,阻碍了全面的分析.
研究的目的:
- 创建一个集中,策划和标准化的数据库,记录人类细胞系对压力的转录性反应.
- 促进对保存和压力特定途径,RNA生物标志物和疾病机制的研究.
主要方法:
- 整合和标准化来自基因表达大盘 (GEO) 的大量RNA测序 (RNA-seq) 数据集.
- 专注于信使RNA (mRNA) 和长非编码RNA (lncRNA) 在氧化应激,缺氧,热冲击和DNA损伤下表达.
- 开发一个统一的计算管道,用于交叉研究数据的可比性和详细的元数据注释.
主要成果:
- 现在可以使用压力反应活动图谱 (ASTRA) 数据库,对各种压力条件的转录数据进行分类.
- 该数据库包括压力和匹配的对照样本,并附有全面的注释 (细胞系,组织来源,细胞状态,治疗,协议).
- 用户可以查询基因表达,执行差异表达分析,并可视化转录动态.
结论:
- 在压力下探索编码和非编码转录组的ASTRA是一个全面的资源.
- 它可以发现新的应激反应基因和RNA介导的调节机制.
- 该数据库支持功能基因组学,系统生物学和细胞适应和病变发生的翻译研究.
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