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Updated: Jun 11, 2026

Force Spectroscopy of Single Protein Molecules Using an Atomic Force Microscope
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Force Spectroscopy of Single Protein Molecules Using an Atomic Force Microscope

Published on: February 28, 2019

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对于单分子力光谱的OaAEP1依赖的酶性蛋白质结合和固定

Lichao Liu1, Peng Zheng1

  • 1State Key Laboratory of Coordination Chemistry, School of Chemistry and Chemical Engineering, Chemistry and Biomedicine Innovation Centre (ChemBIC), Nanjing University, Nanjing, P. R. China. pengz@nju.edu.cn.

Chemical communications (Cambridge, England)
|November 20, 2025
PubMed
概括

使用工程化阿斯巴拉基尼尔内酶OaAEP1的酶介导蛋白质结合使无痕键形成. 这一突破通过提高特异性和效率,推进了单分子力谱学和蛋白质纳米技术.

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Covalent Immobilization of Proteins for the Single Molecule Force Spectroscopy

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OaAEP1-Mediated Enzymatic Synthesis and Immobilization of Polymerized Protein for Single-Molecule Force Spectroscopy

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相关概念视频

Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...