马病毒Y外套蛋白在细胞间运动动态中的功能是由其N端区域定义的
Anže Vozelj1,2, Tjaša Mahkovec Povalej1, Katja Stare1
1Department of Biotechnology and Systems Biology, National Institute of Biology, Ljubljana, Slovenia.
Journal of virology
|November 20, 2025
概括
皮层蛋白 (CP) 的N端区域对于土豆病毒Y (PVY) 运动至关重要. 从PVY CP N端删除特定的氨基酸会显著损害或阻止其在植物中细胞间和系统传播.
科学领域:
- 植物病毒学 植物病毒学
- 分子植物病理学 分子植物病理学
- 植物与病毒的相互作用
背景情况:
- 马病毒Y (PVY) 是一种主要的具有经济意义的植物病毒,在Solanaceae作物中造成大量的产量损失.
- PVY感染会对茎质量和产量产生负面影响,特别是在土豆中.
- 以前的研究表明,PVY外套蛋白 (CP) 的N端区域在病毒传播中起着作用.
研究的目的:
- 确定PVY外套蛋白 (CP) 的特定区域,这对于有效的细胞间和全身运动至关重要.
- 了解宿主植物内PVY传播的基础分子机制.
- 为制定管理PVY感染和减轻产量损失的战略做出贡献.
主要方法:
- 构建和分析CP的PVY N-终端删除突变体.
- 在Nicotiana clevelandii植物中进行感染测定,以评估PVY的繁殖和移动.
- 在CPN终端区域内引入点突变,以评估它们对PVY运动的影响.
主要成果:
- 从CPN终端删除40个或更多的氨基酸,限制了PVY乘法到初级感染细胞.
- 删除26个残留物严重损害了PVY细胞对细胞的运动,并防止了系统性传播.
- 删除19-23残留物或特定点突变 (S21G,G20P) 导致PVY运动延迟.
结论:
- PVY外套蛋白的N端对于有效的病毒运动至关重要.
- 在CPN末端内的特定氨基酸残留物在调节PVY细胞间和全身传播方面发挥着独特的作用.
- 了解这些CP介导的运动功能是控制作物中PVY感染的关键.
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