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综合生物信息学分析以阐明乳腺癌微环境中的细胞通信

Man Wang¹, Hua Li^2,3,4, Xingong Zhang⁴

¹Taihe Hospital, Hubei University of Medicine, Shiyan, Hubei, China.

Discover oncology

|November 20, 2025

概括

乳腺癌的进展受到瘤微环境 (TME) 内的细胞通信的影响. 关键的通路如CXCL12-CXCR4和FGF7-FGFR1,以及CHRDL1,SCARA5,LYVE1,PI16和SAA2的基因,都是至关重要的. 这些因素也会影响免疫细胞的透.

科学领域:

在瘤学瘤学.
生物信息学是一种生物信息学.
免疫学免疫学免疫学

背景情况:

乳腺癌 (BC) 是一种常见的恶性瘤,具有复杂的瘤微环境 (TME).
在BC TME内的细胞间通信显著影响瘤进展.
关于BC细胞间通信的综合研究有限.

研究的目的:

通过综合生物信息分析,阐明BC TME中的细胞通信.
为了确定关键的带受体对和与BC TME相关的差异表达基因.
验证临床样本中的计算发现,并评估免疫学相关性.

主要方法:

使用单细胞和散装mRNA测序数据 (GSE176078) 进行BC TME分析.
确定了关键的细胞通信对 (CXCL12-CXCR4,FGF7-FGFR1) 和最高差异表达基因 (CHRDL1,SCARA5,LYVE1,PI16,SAA2).
在临床样本中通过qPCR验证基因表达,并在单细胞分辨率下分析免疫细胞透.

主要成果:

在BC TME中,CXCL12-CXCR4和FGF7-FGFR1被确定为关键通信对.
CHRDL1,SCARA5,LYVE1,PI16和SAA2成为与这些对相关的显著差异性表达基因.
在临床样本中验证了基因表达趋势,并发现了与免疫细胞透的显著关联.

结论:

在BC TME中的细胞间通信,特别是CXCL12-CXCR4和FGF7-FGFR1通路,加剧了癌症的发展.
不同表达的基因CHRDL1,SCARA5,LYVE1,PI16,SAA2在BC TME机制中发挥着关键作用.
这些已识别的基因和通路与免疫细胞透有显著联系,塑造了BC TME.

关键词:

乳腺癌是什么? 乳腺癌是什么大量的mRNA序列.细胞与细胞之间的通信.单细胞测序是一种单细胞测序.瘤的微环境瘤的微环境

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