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假定肌肉干细胞通过修改巨细胞功能通过c1qtnf3促进Xenopus尾部再生

Sumika Kato¹, Takeo Kubo¹, Taro Fukazawa¹

¹Department of Biological Sciences, Graduate School of Science, The University of Tokyo, Bunkyo-ku, Tokyo 113-0033, Japan.

Proceedings of the National Academy of Sciences of the United States of America

|November 20, 2025

概括

在Xenopus laevis幼尾部再生中,肌肉干细胞使用补充C1q瘤亡因子相关蛋白3 (C1QTNF3) 调节巨细胞积累,这对于成功的再生至关重要.

科学领域:

再生生物学再生生物学
干细胞研究的研究.
克塞诺普斯 (Xenopus laevis) 是一个模型生物.

背景情况:

在Xenopus laevis幼尾部再生中的组织干细胞动态尚不清楚.
之前的工作使用侧面群体 (SP) 方法丰富了干细胞/原始细胞.

研究的目的:

识别和表征组织干细胞群体,在尾部再生过程中启动分化.
研究特定基因在干细胞行为和再生中的作用.

主要方法:

单细胞RNA测序 (scRNA-seq) 的SP部分.
轨迹推断到模型差异化路径.
基于c1qtnf3.3.的CRISPR/Cas9的淘汰 (KD) 的c1qtnf3.
救援实验涉及强迫基因表达在巨类细胞.

主要成果:

已识别的补充C1q瘤亡因子相关蛋白3 (c1qtnf3),具体表达在假定肌肉干细胞 (MSC) 中.
证明c1qtnf3对于Xenopus laevis的尾部再生至关重要.
表明c1qtnf3的淘汰会通过破坏巨类细胞积累来损害尾部再生.
救援实验表明巨细胞失调是受损再生的原因.

结论:

假定MSCs通过c1qtnf3表达在Xenopus尾部再生期间调节巨细胞功能.
C1QTNF3在协调干细胞和免疫细胞行为中起着至关重要的作用,以成功修复组织.

关键词:

它们是 Xenopus laevis 的类型.补充c1q瘤亡因子相关的蛋白质3 3 巨细胞是一个巨细胞.复兴再生是一种再生方式.干细胞是干细胞的组成部分.

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