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核酶RNase Z是一种进化保守的deAMPylase

Meghomukta Mukherjee¹, Alex Pon¹, Timea Goldberg¹

¹Department of Physiology, University of Texas Southwestern Medical Center, Dallas, TX 75390.

Proceedings of the National Academy of Sciences of the United States of America

|November 20, 2025

概括

研究人员发现,RNase Z从蛋白质中去除腺单酸盐 (AMP),将其确定为可逆蛋白质AMPylation中的关键酶. 这一发现揭示了RNase Z在tRNA处理中的已知功能之外的新角色.

科学领域:

生物化学生物化学
分子生物学分子生物学
细胞的新陈代谢

背景情况:

蛋白质AMPylation是一种保守的翻译后修饰,其中腺单酸盐 (AMP) 附着在蛋白质上.
线粒体AMPylase,Selenoprotein O (SelO),通过蛋白质AMPylation调节新陈代谢和氧化应激.
负责从修饰蛋白中去除AMP的酶以前是未知的.

研究的目的:

为了确定催化AMPylated蛋白质基质的deAMPylation的酶.
阐明这种酶在可逆AMPylation途径中的作用.
了解RNase Z. 的更广泛的生物学意义.

主要方法:

生物化学试验测试deAMPylation活动.
酶动力学研究.
在体外和体内生物功能测定.

主要成果:

核糖酶Z (RNase Z) 被确定为负责deAMPylation的酶.
RNase Z既必要又足以从AMP基质中去除AMP.
这确立了RNase Z作为一个月光酶,具有超越tRNA处理的新功能.

结论:

RNase Z催化了deAMPylation,揭示了它在可逆蛋白质AMPylation中的作用.
这一发现凸显了AMPylation作为调节机制的重要性,类似于酸化.
RNase Z具有以前未知的生物功能,扩大了其已知的意义.

关键词:

在AMPylation的过程中,AMPylation的细胞内核酶 (endonuclease) 是一种细胞内核酶.RNase BN BN 的意思是腺化是什么意思?单蛋白 O 是一种单蛋白.

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