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Updated: Jun 14, 2026

Metabolic Pathway Confirmation and Discovery Through 13C-labeling of Proteinogenic Amino Acids
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针对C稳定同位素标记用均标记的葡萄糖和谷氨酸的向代谢方法,使用液体染色学-并发质谱法 (LC-MS/MS)

Erin Q Jennings1,2, W Kimryn Rathmell2, Jeffrey C Rathmell3,4

  • 1National Institute of Digestive and Diabetes and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892, United States.

Journal of proteome research
|November 20, 2025
PubMed
概括

重碳标签有助于代谢疾病研究. 一种新的向质谱法优化了对糖解和相关途径的13C标记分析,改善了在疾病中的葡萄糖和谷氨酸利用研究.

关键词:
碳追踪是指碳的追踪.稳定同位素标记的标签双联质谱质量测量技术有针对性的代谢组学.

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Mass Spectrometry: Complex Analysis01:21

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Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...