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肝脏cyp24a1作为生物标志物用于诊断尼罗河鱼 (Oreochromis niloticus) 暴露于聚烯微塑料中的生物标志物

Feilong Wang¹, Haiyan Xu¹, Xiaomeng Guo¹

¹Anhui Province Key Laboratory of Pollution Damage and Biological Control for Huaihe River Basin, Fuyang Normal University, Fuyang, Anhui, 236037, China.

Aquatic toxicology (Amsterdam, Netherlands)

|November 20, 2025

概括

研究人员在尼罗河鱼中发现了一种潜在的生物标志物Cyp24a1,用于检测水生环境中的聚烯微塑料暴露. 这个基因,这个基因.

科学领域:

环境毒理学环境毒理学
分子生物学分子生物学
水生生态毒理学水生生态毒理学

背景情况:

微塑料 (MPs),特别是聚钢 (PS),是全球水生污染物.
环境监测需要有效的生物标志物来诊断鱼类中的PS暴露.
在鱼类中对PS暴露的进化保存的诊断标记不明.

研究的目的:

在尼罗河鱼 (Oreochromis niloticus) 中识别和描述聚乙烯微塑料暴露的潜在生物标志物.
研究鱼类对PS暴露的转录和蛋白质水平反应.
为了评估在teleosts内识别的生物标志物的跨物种保护.

主要方法:

对88个细胞染色体P450 (CYP) 基因进行全基因组和多组织转录组分析.
在急性,适应性和亚慢性PS暴露后,尼罗鱼肝脏组织的RNA测序.
使用qPCR,西部斑点,免疫光和家族遗传分析验证候选生物标志物.

主要成果:

聚乙烯暴露诱导肝细胞核皮克诺斯和失调的CYP基因表达.
细胞染色体P450通路发生了显著的改变,Cyp24a1在所有暴露阶段都显示了持续的上调.
蛋白质Cyp24a1定位在肝脏脂肪细胞中,并被发现在14种teleost物种中保存.

结论:

肝炎Cyp24a1被提议作为一种潜在的生物标志物,用于诊断鱼类的聚烯微塑性暴露.
早期和持续的Cyp24a1诱导表明它对于环境监测微塑料污染的有用性.
这项研究为开发水生微塑料污染的敏感诊断工具提供了基础.

关键词:

发现生物标志物的发现.细胞染色体P450的使用.尼罗河鱼是尼罗河鱼.聚乙烯微塑料的微塑料.

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