蛋白质组学揭示了牛体内成熟的屠宰场衍生的卵子和体内成熟的卵子之间的实质性差异
Rebecca Herbicht1, Bruno Joaquin Paredes Osorio2, Claudia Klein3
1Institute of Farm Animal Genetics, Friedrich-Loeffler-Institut, Neustadt, Germany.
Animal reproduction science
|November 20, 2025
概括
试管成熟显著改变牛卵细胞蛋白质组,影响发展潜力. 了解这些分子差异是改善体外胚胎生产 (IVP) 成功率的关键.
科学领域:
- 生殖生物学 生殖生物学
- 蛋白质组学是指蛋白质组学.
- 胚胎学 胚胎学
背景情况:
- 在体外生产 (IVP) 牛胚胎面临的局限性包括较低的胚芽细胞数量和胚胎质量比体内生产的胚胎.
- 之前的研究经常使用屠宰场卵子用于IVP,由于卵子来源不同,使得与体内胚胎的比较混乱.
- 成熟环境对IVP和体内胚胎之间的卵细胞蛋白质组差异的影响需要澄清.
研究的目的:
- 为了比较来自不同成熟环境的牛卵细胞的蛋白质组:来自屠宰场材料的体外成熟 (IVM),未成熟的屠宰场材料和体内成熟的卵细胞.
- 确定蛋白质组的差异及其对后续胚胎发育和怀孕率的潜在影响.
主要方法:
- 使用SWATHTM-MS对来自三个群体的卵细胞进行蛋白质组分析:未成熟 (屠宰场),体外成熟 (屠宰场) 和体外成熟 (捐赠牛).
- 每组分析了四个技术复制品,每组10个卵细胞.
- 检测到1208种蛋白质,其中160种蛋白质在组对比中显示出不同丰度.
主要成果:
- 卵细胞成熟环境是蛋白质组差异的主要驱动因素.
- 在体外成熟的卵细胞显示出独特的蛋白质组,其特点是能量代谢,转录,翻译和卵细胞激活途径中的增强的翻译活性.
- 在体内成熟涉及自和亡等途径的有组织调节,与体内无定向蛋白质丰度变化形成鲜明对比.
结论:
- 试管成熟显著改变牛卵细胞蛋白质组,导致放松调节,可能会影响后续的发育事件.
- 在体外成熟卵细胞的独特蛋白质特征可以解释IVP中的胚胎质量降低和怀孕率降低.
- 需要进一步研究优化体外成熟方案,以提高牛胚胎发育能力.
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