重新利用细菌溶解物:为预防呼吸道感染而设计可吸入的虫豆微粒
Joana Pinto da Silva1, Melibea Berzosa2, Lucas Chiarentin3
1Centre for Marine Sciences (CIMAR-LA), Universidade do Algarve, Faro, 8005-139, Portugal.
International journal of biological macromolecules
|November 20, 2025
概括
由豆 (LBG) 和细菌溶解物 (BL) 制成的可吸入微粒显示出对抗呼吸道感染的肺免疫有希望. 这些基于LBG的微粒有效地提供细菌抗原,以增强免疫反应.
科学领域:
- 生物材料科学 生物材料科学
- 免疫学 免疫学 免疫学
- 制药科学 制药科学
背景情况:
- 呼吸道感染对全球健康造成重大负担,需要新的预防策略.
- 肺免疫提供了一条直接的途径,在呼吸道中引起局部免疫反应.
- 细菌溶解物 (BL) 含有抗原,对于诱导对细菌病原体的保护性免疫至关重要.
研究的目的:
- 使用豆 (LBG) 和BL用于肺免疫,开发可吸入的微粒.
- 评估LBG-BL微粒的物理化学特性,抗原保存和体外性能.
- 评估LBG-BL微粒作为预防呼吸道感染的配方的潜力.
主要方法:
- 使用喷雾干燥制造的微粒具有不同的LBG:BL比率.
- 分析了形态,空气动力学和粘膜性质的特性.
- 评估了抗原的保存,体外释放动力学和细胞毒性.
主要成果:
- 喷雾干燥的LBG-BL微粒表现出具有高BL关联效率 (高达81%) 的卷曲形态.
- 在10:0.2的LBG:BL比率下,可以获得最佳的空气动力学性能 (4.6μm MMAD,29% FPF).
- 抗原保持保存,释放持续 (85%到24小时),细胞毒性低.
结论:
- 基于LBG的微粒是可吸入BL输送的合适载体.
- 开发的配方显示了针对呼吸道病原体进行有效的肺免疫的潜力.
- 进一步调查LBG-BL微粒用于预防呼吸道感染是有必要的.
相关概念视频
Physical Methods for Controlling Microbial Growth: Radiation and Filtration
Radiation and filtration are essential tools for microbial control, targeting microorganisms through distinct mechanisms. Radiation eliminates microbes by damaging their DNA, either killing them or inhibiting their growth. Based on wavelength, radiation is classified into two types: nonionizing and ionizing radiation.Non-ionizing radiation, such as UV radiation (200–400 nm), is absorbed by DNA, causing defects that effectively disinfect surfaces, air, and water, including safety cabinets.
Microbes in the Production of Fermented Foods
Lactic acid bacteria (LAB) and molds are instrumental in fermenting plant-based foods to enhance preservation and ensure year-round availability. These microbial processes convert plant carbohydrates into organic acids and other metabolites that inhibit spoilage organisms and contribute to the sensory qualities of the final product.In sauerkraut production, cabbage goes through a microbial succession that starts with cocci such as Leuconostoc mesenteroides. These microbes begin fermentation by...
Production of Biopesticides
Biopesticides offer a sustainable alternative to chemical pesticides, utilizing microbial agents to control agricultural pests. Bacillus thuringiensis (Bt) is a widely employed bacterium known for its potent insecticidal activity. Bt biopesticides are favored for their specificity to insect pests, minimal environmental impact, and natural degradability.Mechanism of Bt Toxin Action Bt produces insecticidal crystal (Cry) proteins during its sporulation phase. These proteins form parasporal...


