揭示了用于siRNA传递的神经元向树突的潜力,使用了芯片上的PNS-CNS
Ana P Spencer1, Miguel Xavier2, Sofia C Guimarães3
1i3S - Instituto de Investigação e Inovação em Saúde, Universidade do Porto, Porto, Portugal; INEB - Instituto de Engenharia Biomédica, Universidade do Porto, Porto, Portugal; Faculdade de Engenharia, Universidade do Porto, Porto, Portugal.
概括
新的可生物降解纳米粒子将siRNA传递给神经元,增强神经系统疾病的轴突再生. 这些向的树突复合体显示出治疗中枢神经系统疾病的前景.
科学领域:
- 纳米医学是一种纳米医学.
- 神经科学是一个神经科学.
- 生物技术是生物技术.
背景情况:
- 神经系统疾病在全球范围内造成了大量的死亡和残疾.
- 有限的轴突再生是治疗这些疾病的一个主要挑战.
- 像siRNA这样的RNA干扰 (RNAi) 疗法面临着传递障碍 (稳定性,细胞吸收).
研究的目的:
- 开发新的,可生物降解的树突性纳米粒子,用于针对性地向神经元传递siRNA.
- 在神经系统疾病中增强轴突再生.
- 为了证明这些纳米载体的中枢神经系统准潜力.
主要方法:
- 可生物降解的树突性纳米颗粒与毒素的神经向性结合域的功能化.
- 在神经元培养和微流体模型中评估树状复合体.
- 评估轴突生长和siRNA传递效率.
- 使用PNS-CNS-on-Chip模型来跟踪树突迁移.
主要成果:
- 针对性的树突复合体在神经元培养中显示出生物相容性和有效的siRNA传递.
- 在微流体模型中观察到轴突生长的显著增强.
- 在PNS-CNS-on-Chip模型中,基复合物成功地从PNS迁移到中枢神经系统神经元.
结论:
- 可生物降解的,针对神经元的树突复合体为神经系统疾病中siRNA输送提供了一个有前途的平台.
- 该研究表明有效的中枢神经系统向和增强的轴突再生.
- 这种方法为神经疾病的创新纳米医学治疗铺平了道路.
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02:30Small interfering RNAs (siRNA)
Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the ATP-dependent...
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