蛋白质基因分析揭示了乳酸脱酶在脊髓损伤后血管修复和功能恢复中的作用

蛋白质基因分析揭示了乳酸脱酶在脊髓损伤后血管修复和功能恢复中的作用

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Manjeet Chopra¹, Sumit Kumar², Nidhi Singh³

¹Department of Pharmacology and Toxicology, National Institute of Pharmaceutical Education and Research, Ahmedabad, Opp. Air Force Station, Palaj, Gandhinagar, Gujarat 382355, India.

ACS chemical neuroscience

|November 20, 2025

概括

脊髓损伤 (SCI) 损害了血管的修复. 这项研究发现乳酸脱酶A (LDHA) 支持SCI后内皮细胞存活,乳酸治疗可能促进恢复.

科学领域:

神经科学是一个神经科学.
生物化学生物化学
血管生物学血管生物学

背景情况:

脊髓损伤 (SCI) 引发了病理过程,包括内皮细胞功能障碍和血管损伤.
了解SCI后的分子变化对于开发有效疗法至关重要.

研究的目的:

使用蛋白质组学识别SCI后改变的分子通路.
研究乳酸脱酶A (LDHA) 在SCI后血管修复中的作用.
探索乳酸治疗作为SCI的潜在治疗策略.

主要方法:

无标签的定量蛋白质组学被用于剖析脊髓组织受伤后的状况.
使用FX-11进行LDHA的药理抑制.
评估内皮细胞功能,血管生成和神经元损伤.
对全身乳酸盐治疗对功能恢复的影响的评估.

主要成果:

乳酸脱酶A (LDHA) 在受伤后7天显著上调 (DPI-7).
抑制LDHA会影响内皮细胞存活,减少增殖,阻碍血管生成,并恶化神经元损伤.
鉴定出LDHA是血管内皮生长因子 (VEGF) -VEGFR2信号传递的调节剂.
系统性乳酸盐的使用抵消了LDHA的抑制作用,并改善了功能恢复.

结论:

在支持内皮细胞存活和SCI后血管修复方面,LDHA起着至关重要的作用.
针对LDHA或使用乳酸补充剂是SCI的有希望的治疗途径.
这项研究突出了SCI的背景下血管修复机制的代谢调节.

关键词:

在VEGF−VEGFR2信号传递中.血管新生是因为血管新生.内皮细胞功能障碍内皮细胞功能障碍乳酸脱酶A是一种乳酸脱酶.脊髓损伤导致的脊髓损伤血管退化血管退化

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