使用分子对接,对环保的氧化铜纳米颗粒对抗癌活性和对Streptococcus mutans的抗菌作用进行评估
Salem S Salem1, Abdullah Yousef2, Ehab S Abd El Hamid3,4
1Department of Botany and Microbiology, Faculty of Science, Al-Azhar University, Nasr City, Cairo, 11884, Egypt.
Scientific reports
|November 20, 2025
概括
使用Mentha spicata提取物合成的氧化铜纳米颗粒 (CuO-NPs) 具有强大的抗菌活性,可以对抗Streptococcus mutans,并有效抑制癌细胞生长. 这些CuO-NP由于其抗微生物和细胞毒性特性,对医疗应用具有前景.
科学领域:
- 纳米技术纳米技术
- 材料科学 材料科学 材料科学
- 生物医学工程 生物医学工程
背景情况:
- 氧化铜纳米颗粒 (CuO-NPs) 具有显著的抗菌特性,使其对各种应用具有价值,特别是在医学中.
- 纳米材料的合成和表征对于理解它们的潜在应用至关重要.
研究的目的:
- 为了合成和表征CuO-NPs使用Mentha spicata树叶提取物.
- 为了评估CuO-NPs对Streptococcus mutans的抗菌疗效.
- 评估CuO-NP对OECM-1癌细胞的抗癌活性.
主要方法:
- 使用FTIR,泽塔电位,DLS,EDX,SEM和UV视光谱学对CuO-NP进行表征.
- 抗菌试验包括抑制区,最小抑制度 (MIC) 和生物膜抑制.
- 细胞毒性测定用于确定针对OECM-1癌细胞的IC50值.
主要成果:
- 合成的CuO-NP显示球形形态,表面电荷为-32.5mV,最大吸收率在260nm.
- CuO-NPs对Streptococcus mutans表现出显著的抗菌活性,其16毫米抑制区为0.5 mg/mL,MIC为0.25 mg/mL.
- 在0.0625 mg/ml的剂量下有效抑制S. mutans生物膜的形成,而不会影响浮游生物的生长.
- 在62.5μg/mL时,CuO-NPs降低了OECM-1癌细胞的95.8%,IC50值为227.3μg/mL.
结论:
- 薄荷子衍生的CuO-NP表现出强大的抗菌和抗癌特性.
- 抗菌机制可能涉及与二水酶合成酶 (DHPR) 的相互作用.
- 这些发现突显了CuO-NP作为医学和牙科治疗剂的潜力.
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