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相关概念视频

Bioreactor Design and Operational System01:29

Bioreactor Design and Operational System

151
Bioreactors are engineered vessels designed to cultivate microorganisms under controlled conditions for industrial bioprocessing. They maintain sterility and allow precise regulation of pH, temperature, oxygen, and nutrient levels to optimize microbial growth and metabolite production. Bioreactors range from small laboratory units of 1 liter to industrial systems holding up to 500,000 liters, though only about 75% of their volume is actively used for fermentation. The remaining headspace...
151
Scale-Up Processes01:14

Scale-Up Processes

90
The scale-up of microbial fermentation processes is essential in industrial biotechnology, allowing the transition from laboratory-scale experiments to commercial-scale production while aiming to maintain product yield and quality. This process requires meticulous adjustment of equipment design, process parameters, and contamination control strategies to accommodate increasing culture volumes.At the laboratory scale, cultures are typically maintained in 1 to 10-liter glass or autoclavable...
90
Upstream Processing01:27

Upstream Processing

88
Upstream processing represents a critical phase in biomanufacturing, wherein biological systems such as microorganisms, mammalian cells, or insect cells are cultivated to produce therapeutic proteins, vaccines, enzymes, or other biologically derived products. This phase encompasses all steps from the selection and genetic manipulation of the production organism to the cultivation of cells in bioreactors under tightly controlled environmental conditions.Host Selection and Genetic OptimizationThe...
88
Production of Antibiotics01:27

Production of Antibiotics

174
Penicillin, one of the earliest and most widely used antibiotics, is produced industrially by the filamentous fungus Penicillium chrysogenum. Large stirred-tank bioreactors ranging from tens to hundreds of thousands of liters maintain tightly controlled temperature, pH, and dissolved oxygen conditions to support fungal metabolism and maximize antibiotic yield. Penicillin is a secondary metabolite, synthesized primarily during the stationary growth phase, which requires a carefully managed...
174
Production of Pharmaceuticals01:30

Production of Pharmaceuticals

70
Industrial insulin production uses genetically engineered E. coli expressing a proinsulin gene controlled by a tryptophan promoter and containing a methionine linker for later cleavage. The cells also carry ampicillin resistance for selective growth. Seed cultures are stored at −80 °C and production begins by thawing a small amount to inoculate starter cultures, which are progressively scaled to a 50,000-L bioreactor. In the bioreactor, E. coli grow in nutrient-rich media under...
70
Production of Biopesticides01:18

Production of Biopesticides

85
Biopesticides offer a sustainable alternative to chemical pesticides, utilizing microbial agents to control agricultural pests. Bacillus thuringiensis (Bt) is a widely employed bacterium known for its potent insecticidal activity. Bt biopesticides are favored for their specificity to insect pests, minimal environmental impact, and natural degradability.Mechanism of Bt Toxin Action Bt produces insecticidal crystal (Cry) proteins during its sporulation phase. These proteins form parasporal...
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相关实验视频

Updated: Apr 15, 2026

Single-cell Analysis of Bacillus subtilis Biofilms Using Fluorescence Microscopy and Flow Cytometry
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用Bacillus subtilis进行表面素生产的基于模型的工艺设计.

Eric Hiller1, Manuel Off2, Holger Dittmann2

  • 1Department of Bioprocess Engineering, Institute of Food Science and Biotechnology, University of Hohenheim, Stuttgart, Germany. eric.hiller@uni-hohenheim.de.

AMB Express
|November 20, 2025
PubMed
概括

这项研究开发了一种Bacillus subtilis食批次发酵的动力模型,以优化表面素的生产. 该模型准确地描述了生物质,基质和产品动态,从而改善了工艺设计和更高的产量.

关键词:
这种细菌是 Bacillus subtilis.生物工艺工程是生物工艺工程.生物反应器是一种生物反应器.高细胞密度的细胞密度很高.动态建模 动态建模在表面上发现了表面活性.

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相关实验视频

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Single-cell Analysis of Bacillus subtilis Biofilms Using Fluorescence Microscopy and Flow Cytometry
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科学领域:

  • 工业生物技术 工业生物技术
  • 生物工艺工程 生物工艺工程
  • 微生物生理学 微生物生理学

背景情况:

  • 细菌细菌是一种关键的工业微生物,但其料批发发酵动力学尚不清楚.
  • 缺乏生物性能指标,如时间变化的生产产量,阻碍了优化.
  • 优化料批处理过程需要对微生物动力学和生物性能有深入的了解.

研究的目的:

  • 为了描述Bacillus subtilis BMV9的料批生物反应器动力学,用于表面素生产.
  • 开发一种描述生物质,基质,表面素和酸盐动态的动态模型.
  • 利用动力模型来设计和实施一个优化的,基于模型的料批处理过程.

主要方法:

  • 使用Bacillus subtilis BMV9的料批生物反应器培养物.
  • 一个使用第一阶普通微分方程的动力模型被开发出来.
  • 该模型整合了Monod生长动力学,基质消耗,表面素合成和酸盐形成.
  • 该模型使用12个食批量实验的数据进行了参数化.

主要成果:

  • 运动模型准确地描述了生物质积累,基质消耗和表面素生产率.
  • 该模型提供了对溢出代谢和酸盐形成的见解.
  • 一个基于模型的工艺设计,省略了批量阶段,实现了 46.33 g/L 的产品位.
  • 实现了2.11g/L*h的时空收益率.

结论:

  • 开发的动力模型显著提高了对Bacillus subtilis养批量培养的过程理解.
  • 该模型允许计算非分析可访问的过程参数.
  • 灵敏度分析确定了影响模型输出的关键参数,指导了进一步的优化工作.