Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Labeling DNA Probes03:31

Labeling DNA Probes

9.2K
DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
9.2K
Real Time RT-PCR02:57

Real Time RT-PCR

64.4K
Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...
64.4K
FISH - Fluorescent In-situ Hybridization02:07

FISH - Fluorescent In-situ Hybridization

23.7K
Fluorescence in situ hybridization, or FISH, was developed in the early 1980s and has quickly become one of the most widely used techniques in cytogenetics. Labeled probes are used to bind complementary DNA or RNA sequences on a chromosome or in a region within a cell. Earlier, the probes could only be obtained by cloning or reverse transcription of a DNA template. Currently, the probe oligonucleotides can be synthesized synthetically. Additionally, with the advancement of optical techniques,...
23.7K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Essential Contributions of Ribose and Nucleobases to the Nucleophilic Reactivity of RNA 2'-OH Groups.

Chemistry (Weinheim an der Bergstrasse, Germany)·2026
Same author

Protocol for covalent RNA labeling by RiboLight dyes for detection by in-gel fluorescence and fluorescence microscopy.

STAR protocols·2026
Same author

Correction to "DNA Content and DNA Damage in Raw and Heat-Processed Foods".

Journal of agricultural and food chemistry·2026
Same author

Protocol for covalent RNA labeling by RiboLight dyes for detection by in-gel fluorescence and fluorescence microscopy.

STAR protocols·2026
Same author

Optical Properties of New Donor-Acceptor Dyes for RNA Imaging: Insights from Ab Initio and Hückel's Model Calculations.

The journal of physical chemistry. B·2026
Same author

C‑Nucleosides Stabilize RNA by Reducing Nucleophilicity at 2'-OH.

ACS central science·2025
Same journal

Linker Engineering toward NIR-II Metal-Organic Framework with Maximal Emission beyond 1000 nm for Inflammatory Bowel Disease Imaging.

Journal of the American Chemical Society·2026
Same journal

Observing Kinetic Selectivity in Anthracene Photodimerization through Selective Quenching by Excited States of Proximate Rare Earth Cations.

Journal of the American Chemical Society·2026
Same journal

Sequence-Dependent Folding of Recognition-Encoded Melamine Oligomers.

Journal of the American Chemical Society·2026
Same journal

Large Thermo- and Mechanosalient Actuation via Cooperative Twist Elasticity-Induced Packing Motif Conversion.

Journal of the American Chemical Society·2026
Same journal

Discovery and Biosynthesis of Lanthipeptides Featuring an Azepinoindole Scaffold by Radical <i>S</i>-Adenosylmethionine Enzyme-Catalyzed C-C Bond Formation.

Journal of the American Chemical Society·2026
Same journal

Enantiopurity-Controlled Magnetism in a Two-Dimensional Organic-Inorganic Material.

Journal of the American Chemical Society·2026
查看所有相关文章

相关实验视频

Updated: Jan 10, 2026

Real-time Imaging of Single Engineered RNA Transcripts in Living Cells Using Ratiometric Bimolecular Beacons
12:20

Real-time Imaging of Single Engineered RNA Transcripts in Living Cells Using Ratiometric Bimolecular Beacons

Published on: August 6, 2014

12.2K

基共价探针用于RNA

Jinwoo Shin1, Moon Jung Kim1, Eric T Kool1

  • 1Department of Chemistry, Stanford Cancer Institute, Stanford University, Stanford, California 94305, United States.

Journal of the American Chemical Society
|November 21, 2025
PubMed
概括
此摘要是机器生成的。

研究人员开发出新的光探针,可选择性地与RNA结合,克服现有染料的局限性. 这种共价标记方法可以在各种生物环境中增强RNA成像和分析.

更多相关视频

Split Hybridization Probe Utilizing a DNA Fluorescent Light-up Aptamer as a Signal Reporter for Sequence-Specific Nucleic Acid Analysis
07:10

Split Hybridization Probe Utilizing a DNA Fluorescent Light-up Aptamer as a Signal Reporter for Sequence-Specific Nucleic Acid Analysis

Published on: July 8, 2025

969
Fluorescent End-Labeling and Encapsulation of Long RNAs for Single-Molecule FRET-TIRF Microscopy
10:59

Fluorescent End-Labeling and Encapsulation of Long RNAs for Single-Molecule FRET-TIRF Microscopy

Published on: October 18, 2024

1.2K

相关实验视频

Last Updated: Jan 10, 2026

Real-time Imaging of Single Engineered RNA Transcripts in Living Cells Using Ratiometric Bimolecular Beacons
12:20

Real-time Imaging of Single Engineered RNA Transcripts in Living Cells Using Ratiometric Bimolecular Beacons

Published on: August 6, 2014

12.2K
Split Hybridization Probe Utilizing a DNA Fluorescent Light-up Aptamer as a Signal Reporter for Sequence-Specific Nucleic Acid Analysis
07:10

Split Hybridization Probe Utilizing a DNA Fluorescent Light-up Aptamer as a Signal Reporter for Sequence-Specific Nucleic Acid Analysis

Published on: July 8, 2025

969
Fluorescent End-Labeling and Encapsulation of Long RNAs for Single-Molecule FRET-TIRF Microscopy
10:59

Fluorescent End-Labeling and Encapsulation of Long RNAs for Single-Molecule FRET-TIRF Microscopy

Published on: October 18, 2024

1.2K

科学领域:

  • 生物化学
  • 分子生物学
  • 化学生物学

背景情况:

  • 目前的非共价RNA染料缺乏对RNA和DNA的选择性.
  • 现有的染料表现出弱目标相互作用和高背景信号,限制细胞RNA染色.
  • 需要改进RNA特定标签策略.

研究的目的:

  • 为选择性RNA标记和成像开发一种新的,独立于序列的方法.
  • 创建具有增强的选择性和信号放大RNA分析的光探针.
  • 证明共价标签在生物应用中的实用性.

主要方法:

  • 与RNA 2'-基 (2'-OH) 组的供体-受体基的阿西利米达介导反应.
  • 开发可调节波长的反应式探针,用于共价光附着.
  • 在水的条件下测试探头性能,凝,溶液和活细胞.

主要成果:

  • 在RNA标签上实现了高达390倍的光增强.
  • 对于RNA与DNA的选择性高达970倍.
  • 记录了四种不同的发射颜色用于多功能成像应用.
  • 成功应用了共价基平台用于RNA特异性分析和成像.

结论:

  • 开发的化共价标签方法在RNA检测方面取得了重大进展.
  • 这种方法为RNA成像提供了增强的选择性,信号放大和多功能性.
  • 共价基平台代表了在各种生物环境中进行RNA研究的强大新工具.