将生物衍生DNA转化为生物技术
Wynter A Paiva1, Matthew E Currier1, Samuel E Ashooh1
1Department of Chemistry, College of Engineering and Physical Science, University of New Hampshire, 23 Academic Way, Parsons Hall, Durham, NH 03824.
概括
对生物技术来说,大规模生产转基因是具有挑战性的,但至关重要. 本综述探讨了用于先进应用的可扩展的生物衍生DNA采购,修改和净化方法.
科学领域:
- 生物技术是生物技术.
- 材料科学 材料科学 材料科学
- 分子生物学分子生物学
背景情况:
- DNA技术正在进步,使得更大规模的功能性DNA基材料成为可能.
- 目前在毫克尺度上生产化学改造的DNA是昂贵的,并限制了工业用途.
研究的目的:
- 审查可扩展的DNA采购,修改和净化新兴策略.
- 将生物衍生DNA来源与传统合成方法进行比较.
- 确定在规模上修改生物衍生核酸的实用方法.
主要方法:
- 生物衍生DNA来源 (菌体,等离子体,基因组) 与合成DNA的比较.
- 对生物衍生DNA的化学和酶修饰的文献综述.
- 讨论可扩展的净化和表征技术.
主要成果:
- 生物衍生DNA为大规模应用提供了昂贵合成DNA的替代方案.
- 特定的化学和酶反应适用于修改生物衍生核酸.
- 可扩展的净化方法对于高吞吐量工作流程至关重要.
结论:
- 新兴的策略有助于在材料和生物技术相关的规模生产DNA.
- 在下一代应用中,生物衍生双链DNA (dsDNA) 的更广泛使用是可以实现的.
- 克服生产挑战是释放基于DNA的材料潜力的关键.
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