生物信息学对乳腺癌的TMPO-AS1-let-7b-5p-ESPL1/E2F8监管轴的见解
Rajeev Nema1, Prerna Vats1, Aditi Singh1
1Department of Biosciences, Manipal University Jaipur, Jaipur, Rajasthan, India.
Frontiers in cell and developmental biology
|November 21, 2025
概括
额外的螺旋杆体像1 (ESPL1) 在乳腺癌 (BC) 中过度表达,与生存率差相关. 这种致癌网络涉及E2F8,TMPO-AS1和hsa-let-7b-5p,其中hesperidin是潜在的治疗标.
科学领域:
- 在瘤学瘤学.
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
背景情况:
- 乳腺癌 (BC) 是女性癌症相关死亡的主要原因.
- 额外的螺旋杆体像1 (ESPL1),一个线粒调节器,在各种癌症中经常过度表达.
- 在BC中ESPL1的特定作用和预后意义在很大程度上仍未确定.
研究的目的:
- 为了研究乳腺癌中ESPL1的表达模式.
- 为了确定ESPL1在BC患者生存结果中的预后值.
- 阐明在BC中涉及ESPL1的分子机制和监管网络.
主要方法:
- 通过多种生物信息学工具 (TIMER 2.0,UALCAN,GEPIA2等) 使用TCGA数据挖掘. 将瘤中的ESPL1表达与正常组织进行比较.
- 使用Kaplan-Meier Plotter数据库分析了ESPL1表达和患者存活率 (OS,DFS,RFS) 之间的关联.
- 使用miRNet,TACCO,CancerMIRNome和Enrichr数据库调查miRNA和lncRNA与ESPL1的相关性,随后在患者组织中进行实验验证.
主要成果:
- 与正常的邻近组织相比,ESPL1在BC瘤 (4倍增加) 和转移性组织中显著过度表达.
- 在BC患者中,高ESPL1表达与较短的整体存活率,无病存活率和无复发存活率相关.
- 在ER/PR阴性BC中ESPL1和E2F8升高,与lncRNA TMPO-AS1正相关,与miR hsa-let-7b-5p负相关,这直接与ESPL1的3'UTR结合.
结论:
- 乳腺癌中ESPL1是一个重要的瘤基因,与侵袭性疾病和不良预后有关.
- 在ESPL1的监管网络涉及转录因子E2F8, lncRNATMPO-AS1和miRNAhsa-let-7b-5p.
- 赫斯佩里丁对ESPL1具有高亲和力结合,为乳腺癌提供了潜在的新疗法策略.
关键词:
在ESPL1上获得ESPL1证书.在TCGA中,TCGA就是TCGA.这就是TMPO-AS1的原因.乳腺癌 乳腺癌 乳腺癌网络 ceRNA 网络这就是hsa-let-7b-5的意思.转移 转移 转移 转移预后 预后 预后更多相关视频
09:40Author Spotlight: Unveiling the Role of TMOD3 in Platinum Resistance and Immune Infiltration in Ovarian Cancer
Published on: August 2, 2024
3.1K
07:47Author Spotlight: Unveiling Transmembrane Protein Family-Related Markers in Gastric Cancer and Implications for Targeted Therapies
Published on: September 15, 2023
2.1K
相关概念视频
01:22MicroRNAs
3.8K
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
3.8K
01:22MicroRNAs
23.9K
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...
23.9K
03:03mTOR Signaling and Cancer Progression
4.6K
The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
The mTOR pathway or the...
4.6K
02:23Abnormal Proliferation
5.1K
Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
5.1K
01:05Cancer-Critical Genes II: Tumor Suppressor Genes
9.3K
Genes usually encode proteins necessary for the proper functioning of a healthy cell. Mutations can often cause changes to the gene expression pattern, thereby altering the phenotype.
When the function of certain critical genes, especially those involved in cell cycle regulation and cell growth signaling cascades, gets disrupted, it upsets the cell cycle progression. Such cells with unchecked cell cycles start proliferating uncontrollably and eventually develop into tumors.
Such genes that act...
When the function of certain critical genes, especially those involved in cell cycle regulation and cell growth signaling cascades, gets disrupted, it upsets the cell cycle progression. Such cells with unchecked cell cycles start proliferating uncontrollably and eventually develop into tumors.
Such genes that act...
9.3K
02:39lncRNA - Long Non-coding RNAs
9.7K
In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
9.7K