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数字聚合酶连锁反应为基础的便链球菌定量用于慢性肝病中肝纤维化评估

Nomura Nao¹, Haruki Uojima^1,2, Akira Také³

¹Department of Gastroenterology, Internal Medicine, Kitasato University School of Medicine, Sagamihara, Japan.

Hepatology research : the official journal of the Japan Society of Hepatology

|November 21, 2025

概括

便链球菌 spp. 在便中. 用数字PCR测量的负载显示,它有可能成为慢性肝病 (CLD) 肝纤维化非侵入性生物标志物. 然而,年龄和BMI等因素可能会影响它的准确性.

科学领域:

胃肠病学胃肠病学
微生物学微生物学
生物标志物发现发现

背景情况:

慢性肝病 (CLD) 影响全球数百万人.
肝纤维化的非侵入性生物标志物对于患者管理至关重要.
便微生物群的组成越来越多地被认为是CLD相关的.

研究的目的:

确定与便中的Streptococcus spp.相关的临床因素. 在CLD患者中的负载.
评估便中Streptococcus spp.的有用性. 用数字PCR (dPCR) 作为肝纤维化的非侵入性生物标志物.

主要方法:

对146名CLD患者的回顾性分析.
在便中Streptococcus spp.的量化. 使用dPCR和16S rRNA基因原始物的负载.
由于其影响,对质子抑制剂 (PPI) 用户的单独分析.
相关性和接收器操作特征 (ROC) 曲线分析,以评估肝硬化诊断性能.

主要成果:

便链球菌 spp. 在便中. 在PPI使用者和肝硬化患者中,负荷显著更高.
对肝硬化的ROC分析显示AUC为0.728,低于既定标志物.
链球菌 spp. 链球菌 spp. 负载负荷与年龄正相关,与BMI负相关.
在老年患者 (>65岁) 与较低BMI (≤25 kg/m2) 的患者中观察到更高的负荷.

结论:

便链球菌 spp. 在便中. 通过dPCR测量的负载可能会补充现有的非侵入性纤维化标志物.
与宿主相关的因素,如年龄和BMI可能会影响这个生物标志物的诊断准确性.
需要进一步的研究来完善其临床效用.

关键词:

链球菌 spp. 链球菌 spp.慢性肝脏疾病慢性肝脏疾病数字PCR是数字的PCR.肠肝轴肠肝轴肠肝轴肝脏纤维化肝脏纤维化

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