Search research articles

关于 JoVE

概览领导团队博客 JoVE 帮助中心

作者

出版流程编辑委员会范围与政策同行评审常见问题投稿

图书馆员

用户评价订阅访问资源图书馆顾问委员会常见问题

研究

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments 存档

教育

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual 教师资源中心教师网站

使用条款与条件

相关概念视频

Mouse Models of Cancer Study

Mouse Models of Cancer Study

Mice have long served as models for studying human biology and pathology because of their phylogenetic and physiological similarity with humans. They are also easy to maintain and breed in the laboratory, and hence, many inbred strains are now available for research. Studies on mice have contributed immeasurably to our understanding of cancer biology.
The development of transgenic, knockout, and knock-in mice has led to an exponential increase in their use as model organisms in research,...

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序

Same author

HLA-E single-chain trimer shields gene-edited allogeneic CAR T cells from NK cell attack.

Blood advances·2026

Same author

Editorial: A preamble to the pathology of humanized mouse models.

Veterinary pathology·2026

Same author

Immunogenicity and Efficacy of a Trivalent HSV-2 gC2, gD2, gE2 Nucleoside-Modified mRNA-LNP Vaccine Against HSV-1 Eye Infection and Neuroinvasion in Mice.

Vaccines·2026

Same author

Targeted near-infrared imaging utilizing a cathepsin-activated fluorophore for the intraoperative detection of canine insulinoma.

PloS one·2026

Same author

Development of Dirofilaria immitis adult worms in NSG mice, detection of parasite-derived microRNA and comparative analysis of laboratory isolates.

Scientific reports·2026

Same author

Pathology associated with human CAR T cell administration in NOD.Cg-<i>Prkdc</i><sup><i>scid</i></sup><i>Il2rg</i><sup><i>tm1Wjl</i></sup>/SzJ (NSG) mice: A retrospective analysis.

Veterinary pathology·2026

Search research articles

相关实验视频

Updated: Jan 10, 2026

Testing Cancer Immunotherapeutics in a Humanized Mouse Model Bearing Human Tumors

Testing Cancer Immunotherapeutics in a Humanized Mouse Model Bearing Human Tumors

Published on: December 16, 2022

在人性化小鼠的组织水平上研究嵌合的技术.

Arin Cox¹, Esha Banerjee¹, Jillian Verrelle¹

¹Penn Vet Comparative Pathology Core, University of Pennsylvania, Philadelphia, PA.

Veterinary pathology

|November 21, 2025

概括

这项研究验证了标记技术,以区分人类和老鼠细胞在嵌合体小鼠中,这对于理解疾病模型至关重要. 这些方法帮助研究人员识别特定的细胞类型,改善了对人性化小鼠研究的解释.

关键词:

奇梅里克小鼠奇梅里克小鼠人性化的小鼠.免疫组织化学免疫组织化学鼠标模型鼠标模型异国移植异国移植

更多相关视频

Trans-inner Cell Mass Injection of Embryonic Stem Cells Leads to Higher Chimerism Rates

Trans-inner Cell Mass Injection of Embryonic Stem Cells Leads to Higher Chimerism Rates

Published on: May 29, 2018

Intranasal Administration of Recombinant Influenza Vaccines in Chimeric Mouse Models to Study Mucosal Immunity

Intranasal Administration of Recombinant Influenza Vaccines in Chimeric Mouse Models to Study Mucosal Immunity

Published on: June 25, 2015

相关实验视频

Last Updated: Jan 10, 2026

Testing Cancer Immunotherapeutics in a Humanized Mouse Model Bearing Human Tumors

Testing Cancer Immunotherapeutics in a Humanized Mouse Model Bearing Human Tumors

Published on: December 16, 2022

Trans-inner Cell Mass Injection of Embryonic Stem Cells Leads to Higher Chimerism Rates

Trans-inner Cell Mass Injection of Embryonic Stem Cells Leads to Higher Chimerism Rates

Published on: May 29, 2018

Intranasal Administration of Recombinant Influenza Vaccines in Chimeric Mouse Models to Study Mucosal Immunity

Intranasal Administration of Recombinant Influenza Vaccines in Chimeric Mouse Models to Study Mucosal Immunity

Published on: June 25, 2015

科学领域:

免疫学免疫学免疫学
病理学病理学病理学
生物技术是生物技术.

背景情况:

化学老鼠对于研究人类疾病和治疗方法至关重要.
在这些模型中区分人类和小鼠细胞对于准确的数据解释至关重要.
现有的方法可能缺乏对复杂的仿真组织的特异性或广泛适用性.

研究的目的:

验证和应用一套标签技术,以区分人类和小鼠细胞在仿真小鼠.
为识别特定的人类和小鼠细胞子集,包括免疫细胞和CAR T细胞提供参考.
为了提高空间分辨率和解释使用人性化小鼠模型的研究.

主要方法:

已建立广泛的免疫组织化学标志物 (HLA-A,Ku80,hMito) 用于在甲固定式嵌入样本中的一般人体细胞识别.
为特定的人或小鼠免疫细胞子集开发了量身定制的免疫组织化学和现场杂交.
利用人体组织微阵列和人性化小鼠标本进行验证.

主要成果:

HLA-A证明了可靠的膜性免疫活性,用于人类细胞的识别.
当HLA-A没有表达时,Ku80和hMito作为替代标记物.
经过验证的方法允许在嵌合体组织中区分特定的免疫细胞和人类嵌合体抗原受体T细胞.

结论:

经过验证的标签技术为分析模拟鼠标模型提供了强大的框架.
这些方法对病理学家和研究人类化小鼠的研究人员至关重要.
该研究为复杂的嵌合体组织分析的诊断和实验应用提供了实际应用.