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潜在的全基因组测序以确定新生儿中的细菌传播及其修饰剂

Timmy Nguyen¹, Fabian Bürkin¹, Stefany Ayala-Montaño¹

¹Institute for Infection Prevention and Control, Medical Center-University of Freiburg, Freiburg, Germany.

JAMA network open

|November 21, 2025

概括

新生儿重症监护室 (NICU) 的婴儿容易感染多药耐药生物 (MDROs). 全基因组测序确定了传播事件,揭示了增加护士人员和先前使用抗生素可以降低MDRO传播风险.

科学领域:

新生儿科学新生儿科学
传染性疾病传染性疾病
基因组学就是基因组学.

背景情况:

新生儿重症监护室 (NICU) 的婴儿面临着多药耐药生物 (MDRO) 的风险,可能导致侵入性感染.
对MDRO传播的高分辨率分析对于有针对性的感染预防至关重要.

研究的目的:

评估全基因组测序 (WGS) 在确定NICU环境中的MDRO传输链中的有效性.
确定与MDRO集群参与和传播相关的风险因素.

主要方法:

一项前性单中心队列研究包括了434名被录取到III级NICU的婴儿.
整基因组测序和放大片段长度多态性被用来定义MDRO传播.
分析了依赖时间的患者和病房因素,设备使用和抗生素暴露.

主要成果:

超过一半的婴儿 (51.8%) 被至少一个MDRO殖民.
全基因组测序将34.0%的殖民与传播事件联系起来,确定了37个独特的传播集群.
增加全职护士人员和先前使用抗生素与传播风险降低有关,而血管导管的使用增加了风险.

结论:

细菌测序,特别是WGS,可以准确地检测NICU中的细菌传播事件.
多变量分析表明,NICU环境中的细菌传播风险可以通过特定干预来改变.

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