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查和便的向测序用于微生物学确认和药物耐药性确定在松结核病
Tara E Ness1,2, Mangaliso Ziyane3,4, Nontobeko Maphalala5
1Global Tuberculosis Program, Baylor College of Medicine, Houston, Texas, United States of America.
PLOS global public health
|November 21, 2025
概括
检测Paucibacillary结核病中的耐药性是具有挑战性的. 使用下一代测序 (tNGS) 的便样本的有针对性的测序成功检测出Mycobacterium结核病DNA,并提供了涂抹阴性患者的耐药性信息.
科学领域:
- 微生物学 微生物学
- 基因组学就是基因组学.
- 传染性疾病 传染性疾病
背景情况:
- 结核病 (TB) 仍然是全球主要的健康威胁,每年造成数百万人的死亡.
- 耐药结核病对诊断提出了重大挑战,特别是在因细菌负载较低而患有乳头结核病的患者中.
- 目前用于诊断Mycobacterium结核病耐药性的诊断方法往往不足以治疗乳结核病.
研究的目的:
- 评估针对性下一代测序 (tNGS) 在便样本上的实用性,用于诊断乳结核病和检测耐药性.
- 评估基于便的tNGS在埃斯瓦提尼的门诊环境中的可行性.
主要方法:
- 在埃斯瓦蒂尼 (2020-2023) 招募疑似结核病患者的前景.
- 除了标准诊断测试外,收集便和唾液样本.
- 使用牛津纳米孔技术 (ONT) 和Illumina平台提取和针对性下一代测序 (tNGS) 便DNA.
主要成果:
- 在250名参与者中,有85人 (34%) 患有样阴性肺结核.
- 来自21/85 (24.7%) 涂抹阴性患者的便样本为tNGS提供了足够的DNA.
- 便tNGS在14-19%的患者中检测到Mycobacterium结核病DNA,并在12-14%的患者中确定了耐药性突变.
结论:
- 便样本的有针对性的测序是一种可行的方法,用于确认结核病.
- 基于便的tNGS扩大了对结核病患者的检测能力,超出了传统的基于唾液的方法.
- 这种方法为改善结核病诊断和药物耐药性监测在资源有限的环境中提供了一个有希望的策略.
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