载有miRNA的仿生纳米粒子调节肠道微生物,以改善炎症性肠道疾病
Wenjuan Liu1, Jinlong Yang2, Zibo Wei3
1School of Pharmaceutical Sciences, Fudan University, Key Laboratory of Smart Drug Delivery, Ministry of Education, State Key Laboratory of Advanced Drug Formulations for Overcoming Delivery Barriers, Shanghai 201203, China.
Science advances
|November 21, 2025
概括
使用miRNA载入纳米颗粒的工程细菌改善了炎症性肠病 (IBD) 治疗的肠道健康. 这种新的方法增强了细菌的功能和稳定性,为结肠炎提供了一个有前途的治疗策略.
科学领域:
- 微生物学 微生物学
- 纳米技术 纳米技术
- 胃肠病学 胃肠病学
背景情况:
- 肠道微生物群调节是炎症性肠道疾病 (IBD) 的关键策略.
- 目前的IBD治疗方法,如益生菌和便微生物群移植,在安全性和有效性方面存在局限性.
- 需要先进的治疗策略来有效管理IBD.
研究的目的:
- 通过使用miRNA装载的纳米粒子,设计一种益生菌细菌Lactobacillus rhamnosus (LGG).
- 增强LGG的扩散和印尔-3-碳酸的生产,以提高治疗潜力.
- 开发和评估一种基于纳米粒子的新型药物输送系统,用于结肠炎治疗.
主要方法:
- 工程化共生乳杆菌 (LGG) 带有miRNA载荷的仿生纳米粒子.
- 功能化细菌细胞外囊泡 (BEVs) 由LGG与脂质纳米颗粒 (LNPs) 衍生,以创建BEV-LNPs.
- 在模拟生理和胃肠道环境中评估BEV-LNP稳定性.
- 在急性和慢性结肠炎模型中评估了BEV-LNP配方的治疗疗效.
主要成果:
- 与大肠杆菌相比,BEV-LNP显示了对LGG的增强准效率.
- 在模拟生理流体和胃肠道环境中,BEV-LNP表现出优越的稳定性,而不是传统的LNP.
- BEV-LNP配方与5-氨基酸结合,显著降低了炎症,恢复了上皮屏障的完整性,并在结肠炎模型中促进了微生物平衡.
结论:
- 工程化miRNA装载的纳米颗粒增强了IBD治疗的益生菌.
- BEV-LNP提供了针对结肠炎的治疗剂的稳定有效的输送系统.
- 这一策略是通过调节肠道微生物群来改善结肠炎治疗结果的有希望的方法.
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