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氨色度指标用于测量尿酶和尿解细菌度的氨色度指标
Michaella Watson1, Christopher O'Rourke1, Andrew Mills1
1School of Chemistry and Chemical Engineering, Queens University Belfast, Stranmillis Road, Belfast, BT9 5AG, UK.
Biosensors & bioelectronics
|November 21, 2025
概括
新的方法量化尿酶和尿溶性细菌,使用色度氨指示器和数字色彩分析. 这种方法可以准确地测量细菌度和酶活性.
科学领域:
- 生物化学和微生物学
- 分析化学 分析化学
- 酶检测 酶检测 酶检测 酶检测
- 细菌定量测定 细菌定量测定
背景情况:
- 精确确定尿酶活性和尿溶性细菌度在各种科学领域至关重要.
- 现有的方法可能耗时,需要专门的设备,或缺乏对各种条件的敏感性.
- 对于微生物和酶学研究,需要开发快速,经济高效和多功能分析工具.
研究的目的:
- 开发和验证两种用于量化尿酶和尿溶性细菌度的新方法.
- 为了使用不可逆转的色度氨指示器加上数字色彩分析 (DCA) 进行定量测量.
- 建立适用于有氧和无氧条件的方法,以便广泛适用.
主要方法:
- 方法 (i):尿酸酶量化包括将尿素与尿酸酶样本和指示器化,使用DCA监测随时间 (t) 变化的颜色,以确定t50 (半变化时间).
- 方法 (ii):尿溶性细菌量化包括用细菌接种以尿素为基础的介质,添加指示器,并通过DCA监测颜色变化以确定TT (值时间).
- 数字色彩分析 (DCA) 通过测量指示器中的明显吸收率 (A') 变化来量化氨 (NH3) 产量.
主要成果:
- t50的反向是与尿酶度 ([尿酶]) 直接成比例的.
- 值时间 (TT) 与广泛范围 (10^1-10^8 CFU/mL) 的尿溶性细菌度 (log(CFU/mL)) 的对数成正比.
- 基于NH3指标的微呼吸计方法用于总活力计数 (NH3μR-TVC) 即使在有氧和无氧条件下存在非尿溶性物种 (例如大肠杆菌),也能准确量化细菌.
结论:
- 开发的色度测量方法为量化尿素酶和尿溶性细菌提供了一种敏感而有效的方法.
- 数字色彩分析提供了一个强大的平台来解释指示器的响应,从而能够准确地确定度.
- NH3μR-TVC方法是一种用于细菌计数的新多功能工具,适用于各种环境和临床环境.
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