相关实验视频

Updated: Jan 10, 2026

Three-dimensional Inflammatory Human Tissue Equivalents of Gingiva

Three-dimensional Inflammatory Human Tissue Equivalents of Gingiva

Published on: April 3, 2018

纤维素7C终端生物活性通过整合素α5β1调节炎症期间巨细胞的代谢概况

Saloni Gupta¹, Shubham Kumar Rai¹, Rama N Behera¹

¹Department of Biosciences and Bioengineering, Indian Institute of Technology Roorkee, Roorkee, Uttarakhand, 247667, India.

Biochemical and biophysical research communications

|November 21, 2025

概括

酸FC-10通过调节细胞代谢,促进巨细胞的抗炎状态. 它将能量生产从糖解转移到TCA循环和谷氨酸代谢,提供潜在的抗炎治疗益处.

科学领域:

免疫学免疫学免疫学
细胞的新陈代谢
分子生物学分子生物学

背景情况:

炎症会诱导单细胞和巨细胞中的代谢重编程.
纤维素7C终端片段 (Fbln7-C) 和其 (FC-10) 通过整合素α5β1.1.调节免疫细胞功能.
综合素α5β1信号与疾病中的代谢途径调节有关.

研究的目的:

为了研究FC-10,整合素α5β1和炎症期间巨细胞中免疫代谢编程之间的相关性.
阐明巨细胞中由FC-10调节的特定代谢途径.

主要方法:

利用了用脂多糖 (LPS) 刺激的THP-1和主要单细胞衍生的巨细胞.
采用液体染色学合联质谱法 (LC-MS/MS) 用于蛋白质学和代谢学.
进行基因表达分析和整合素α5β1阻断实验.

主要成果:

FC-10促进了一种抗炎M2巨细胞表型 (CD206,IL-10表达).
FC-10 降低了炎症和甘油性蛋白质 (hexokinase,pyruvate kinase),同时增加了 TCA 循环和谷氨酸代谢蛋白质 (酸盐合成酶,GDH1,SLC1A5).
阻断整合素α5β1逆转了这些代谢变化,增加了糖解,减少了谷氨酸代谢.

结论:

在炎症期间,FC-10调节巨细胞的代谢过程,转向抗炎性表型.
FC-10的机制涉及整合素α5β1信号传输.
通过向细胞代谢,FC-10显示出作为一种抗炎治疗剂的潜力.

关键词:

在FC-10中,我们可以看到FC-10.炎症炎症是一种炎症.整合物是一种整合物.巨细胞是一个巨细胞.代谢过程中的代谢.

更多相关视频

A Multi-Cue Bioreactor to Evaluate the Inflammatory and Regenerative Capacity of Biomaterials under Flow and Stretch

A Multi-Cue Bioreactor to Evaluate the Inflammatory and Regenerative Capacity of Biomaterials under Flow and Stretch

Published on: December 10, 2020

Metabolic Characterization of Polarized M1 and M2 Bone Marrow-derived Macrophages Using Real-time Extracellular Flux Analysis

Metabolic Characterization of Polarized M1 and M2 Bone Marrow-derived Macrophages Using Real-time Extracellular Flux Analysis

Published on: November 28, 2015

相关概念视频

Intracellular Signaling Affects Focal Adhesions

Intracellular Signaling Affects Focal Adhesions

Integrins act both as extracellular input receivers and as intracellular processing activators. As their name suggests, integrins are entirely integrated into the membrane structure. Their hydrophobic membrane-spanning regions interact with the phospholipid bilayer's hydrophobic region. These membrane receptors provide extracellular attachment sites for effectors like hormones and growth factors. They activate intracellular response cascades when their effectors are bound and active.
Some...

Fibronectins Connect Cells with ECM

Fibronectins Connect Cells with ECM

Fibronectin is an adhesive glycoprotein present in the extracellular matrix of embryogenic and adult tissue. These molecules primarily aid in regulating cell motility and attachment. A fibronectin molecule is composed of two identical polypeptide chains attached to each other by a pair of disulfide bonds at the C-terminal.
Both proteoglycans and collagen are attached to fibronectin proteins, which, in turn, are attached to integrin proteins. These integrin proteins interact with transmembrane...