整合机器学习与横流免疫试验,用于超快速和灵敏的塔达拉菲尔检测
Jiawei Chen1, Zhenzhen Chen1, Sha Yu1
1State Key Laboratory of Food Science and Resources, Nanchang University, 235 East Nanjing Road, Nanchang 330047, China.
Food chemistry
|November 21, 2025
概括
一种新方法将侧流免疫检测与机器学习相结合,用于快速,准确地在现场检测补充剂中发现的危险药物塔达拉菲尔. 这种方法大大缩短了测试时间,仅为3分钟.
科学领域:
- 分析化学 分析化学
- 生物技术是生物技术.
- 食品安全 食品安全
背景情况:
- 塔达拉菲尔是一种固酶5型抑制剂,在功能性食品和食补充剂中非法存在,造成健康风险.
- 敏感和快速检测方法对于污染产品的现场查至关重要.
研究的目的:
- 开发一种敏感且快速的tadalafil现场检测方法.
- 将侧流免疫试验 (LFIA) 与机器学习 (ML) 集成,以提高检测能力.
主要方法:
- 制备一种高亲和度的抗塔达拉菲尔单克隆抗体 (3C5).
- 使用随机森林模型开发与机器学习 (LFIA@ML) 集成的侧流免疫试验.
- 与液体染色学-并联质谱学 (LC-MS/MS) 进行验证.
主要成果:
- 在140个样本中,LFIA@ML实现了3分钟的检测时间,预测准确率为94.3%.
- 随机森林模型显示了高性能 (R2=0.995,MAE=0.038).
- 试验结果显示与LC-MS/MS有很强的相关性,康复率在92.3%至112%之间.
结论:
- LFIA@ML方法提供了一种快速,准确和可靠的方法,用于现场检测tadalafil.
- 这项技术可以有效地用于选葡萄酒等复杂矩阵中的塔达拉菲尔.
- 整合LFIA与ML显著提高了伪造产品的检测速度和准确性.
更多相关视频
09:58Computer Numerical Control Micromilling of a Microfluidic Acrylic Device with a Staggered Restriction for Magnetic Nanoparticle-Based Immunoassays
Published on: June 23, 2022
2.6K
07:13Author Spotlight: High-Quality Quantum Dot Nanobeads for Sensitive Fluorescent Lateral Flow Immunoassays
Published on: June 28, 2024
2.1K
相关概念视频
Rapid Identification of Pathogens
MALDI-TOF MS has transformed clinical microbiology by offering a rapid and reliable method for pathogen identification. The traditional approach to microbial identification typically involves time-consuming culture techniques and biochemical tests, which can delay the initiation of appropriate antimicrobial therapy. MALDI-TOF MS avoids these delays by using characteristic ribosomal protein mass patterns of microbial cells, enabling accurate species-level identification within minutes.Principle...
Automated Microbial Diagnostics
Automated diagnostic analyzers have transformed clinical microbiology by providing rapid and reliable methods for pathogen identification and antibiotic susceptibility testing. Among these systems, the Vitek 2 is widely used because it automates the traditionally labor-intensive processes of microbial identification (ID) and antibiotic susceptibility testing (AST), delivering standardized and timely results that are essential for effective patient care.Microbial Identification with ID CardsThe...
