在kombucha发酵过程中,物理化学性质,风味和微生物群落的动态变化与主导的本土菌株接种疫苗
1School of Food Science and Engineering, Wuhan Polytechnic University, Wuhan 430023, China; Hubei Key Laboratory for Processing and Transformation of Agricultural Products (Wuhan Polytechnic University), Wuhan 430023, China.
Food research international (Ottawa, Ont.)
|November 21, 2025
概括
这项研究使用定义的微生物菌株优化了kombucha发酵. 三种菌株共同培养 (MS2) 增强了味道和一致性,为工业生产提供了战略.
科学领域:
- 食品科学和微生物学
- 发酵技术的发酵技术
- 感官分析 感官分析
背景情况:
- 传统的kombucha发酵在效率,一致性和微生物控制方面面临着挑战.
- 使用已定义的本土菌株为标准化kombucha生产提供了一个潜在的解决方案.
研究的目的:
- 系统地监测用定义的菌株发酵的康布查的物理化学性质,微生物群落和风味特征.
- 确定最佳的微生物组合,以提高发酵性能和感官属性.
主要方法:
- 在单种和共同培养条件下使用定义的菌株 (Komagataeibacter intermedius,Saccharomyces cerevisiae,Lactobacillus sakei) 进行豆发酵.
- 对物理化学性质 (pH,总酸度) 的分析.
- 高通量测序用于微生物社区分析.
- 电子传感分析和气体色谱-离子运动谱法 (GC-IMS) 用于挥发性风味化合物.
- 斯皮尔曼相关性分析将微生物群落和风味概况联系起来.
主要成果:
- 三重菌株共同培养 (MS2) 显示出最佳的发酵,达到最低的pH值 (2.49) 和最高的总酸度 (3.28 g/L).
- 在发酵后观察到不同的微生物群落,其中Saccharomyces和Komagataeibacter在MS2中占主导地位.
- 在MS2中,以为主要的挥发性化合物 (81.24%),与增强的酸度,丰度和水果/花气息相关.
- 在MS2组中,观察到苦和性的减少.
结论:
- 定义的本土微生物联盟,特别是三种菌株共同培养 (MS2),可以显著提高kombucha发酵效率和一致性.
- 特定的微生物组成直接影响口味特征,从而导致可取的感官属性,如增强的果味.
- 这些发现为选择功能性微生物提供了科学基础,以标准化和提高工业生产的kombucha的风味质量.
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