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克隆,表达和功能性特征的准备牛,鱼和鱼基础纤维细胞生长因子-2的功能性特征

S Skrivergaard¹, M E Pedersen², A Holck³

¹Department of Food Science, Aarhus University, Aarhus, Denmark.

NPJ science of food

|November 21, 2025

概括

研究人员从牛,鱼和鱼中开发出具有成本效益的复合纤维细胞生长因子-2 (FGF-2). 在无血清条件下,牛FGF-2在支持肌肉干细胞 (MuSC) 增殖方面表现出卓越的疗效,降低了整体中等成本.

科学领域:

生物技术是生物技术.
细胞生物学细胞生物学
生物工艺工程生物工艺工程

背景情况:

增长因子 (GFs) 是细胞培养基的重要成本驱动因素,超过总费用的90%.
纤维细胞生长因子2 (FGF-2) 对于支持肌肉干细胞 (MuSC) 增殖和相关信号通路至关重要.

研究的目的:

开发和评估来自牛,鱼和鱼的重组FGF-2变体,以实现成本效益高的MuSC扩张.
评估内部生产的FGF-2在无血清介质中的生物活性和疗效.

主要方法:

牛,鱼和鱼FGF-2的codon优化的基因被克隆成一个大肠杆菌表达载体.
复合FGF-2生物活性在补充了fetuin和胰岛素-转移素- (ITS) 的无血清介质中进行了测试.
在对FGF-2治疗的反应中,分析了MuSC增殖和关键信号通路 (ERK,p38).

主要成果:

所有三种复合FGF-2变异都支持在无血清介质中的MuSC增殖.
与鱼和鱼FGF-2 (17.5-70 ng/mL) 相比,牛FGF-2在较低度 (2 ng/mL) 中表现出更高的疗效.
在牛FGF-2和p38抑制剂之间观察到协同作用.

结论:

公司内部生产复合FGF-2提供了一种可行的策略,以降低细胞治疗制造中的中等成本.
优化牛FGF-2的生产和应用可以显著降低与生长因素相关的费用.
这些发现支持基于细胞的产品制造通过成本降低策略的大规模可行性.

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