相关实验视频
Updated: May 10, 2026

08:06
Y-27632 Enriches the Yield of Human Melanocytes from Adult Skin Tissues
Published on: July 8, 2020
6.0K
可持续的重复批次发酵不动化的Ganoderma formosanum用于增强抗黑色素生物活性化合物的生产
Chen-Che Hsieh1, Yi-Wen Chen2, Yi-Cheng Huang3
1Department of Seafood Science, National Kaohsiung University of Science and Technology, Nanzih District, Kaohsiung, Taiwan, ROC.
NPJ science of food
|November 21, 2025
概括
这项研究开发了使用农业副产品的Ganoderma formosanum mycelia的可持续发酵方法. 这种环保的工艺有效地产生功能性食品的天然抗黑色素生成化合物.
科学领域:
- 生物技术是生物技术.
- 菌类学 菌类学是指菌类学.
- 食品科学 食品科学 食品科学
背景情况:
- 甲菌 (Ganoderma formosanum) 是一种有价值的药用真菌,含有生物活性化合物.
- 传统的种植方法昂贵且耗时,限制了其在功能性食品中的使用.
- 开发可持续和高效的生产方法对于其应用至关重要.
研究的目的:
- 建立一个创新的和可持续的发酵平台,用于Ganoderma formosanum mycelia (GFM).
- 用农业副产品生产功能性食品应用的天然抗黑色素生成化合物.
- 优化菌根固定和发酵条件,以提高产量和效率.
主要方法:
- 在塑料复合材料支 (PCS) 上使用大豆,聚烯和酵母提取物 (SY-PCS) 固定GFM.
- 在空运生物反应堆中优化固定矩阵和扩展.
- 优化发酵过程,包括重复批次栽培.
- 使用基于细胞的测定 (B16-F10) 和斑马鱼模型评估抗黑色素活性.
- 使用LC-MS/MS和分子对接研究识别生物活性化合物.
主要成果:
- 在SY-PCS矩阵被证明是细胞不动化的最佳,在摇瓶中达到79.78%的酸 (KA) 抗黑色素疗效.
- 升级到5L生物反应器将功效提高到KA功率的81.7%.
- 重复批次发酵使第二批生物质的生产率提高了2.7倍.
- 基于细胞的测定和斑马鱼的试验证实了显著的黑色素减少.
- LC-MS/MS确定了15种具有良好的铁酶结合亲缘关系的生物活性化合物.
结论:
- 开发的可持续发酵平台提供了一种生态有效的方法,用于生产G.formosanum衍生的抗黑色素生成化合物.
- 这些化合物显示出作为皮肤健康功能性食品中的天然添加剂的巨大潜力.
- 固定化的GFM系统展示了适合食品级应用的资源效率高的产量.
更多相关视频
相关概念视频
Bioreactor Controls-III
Strain improvement is a foundational strategy in industrial microbiology aimed at maximizing microbial productivity, particularly because natural isolates typically yield commercially valuable products in very low concentrations. Although optimizing the culture medium and environmental conditions can improve yields, these adjustments are inherently limited by the organism’s genetic potential. As a result, the focus shifts toward genetic modifications to enhance biosynthetic capacity. The...
Batch vs Continuous Culture
Fermentation is a foundational biotechnological process used to produce pharmaceuticals, biofuels, enzymes, and food additives. Among industrial strategies, batch and continuous fermentation are the two most widely applied. Although both rely on microbial conversion of substrates into desired products, they differ markedly in operation, productivity, and suitability for specific applications.Batch fermentation occurs in a closed system in which nutrient media and inoculum are added at the...
Upstream Processing
Upstream processing represents a critical phase in biomanufacturing, wherein biological systems such as microorganisms, mammalian cells, or insect cells are cultivated to produce therapeutic proteins, vaccines, enzymes, or other biologically derived products. This phase encompasses all steps from the selection and genetic manipulation of the production organism to the cultivation of cells in bioreactors under tightly controlled environmental conditions.Host Selection and Genetic OptimizationThe...
Production of Organic Acids
Lactic acid, an important organic acid extensively applied in food, pharmaceutical, and biodegradable polymer industries, is primarily produced via microbial fermentation. This method is favored over chemical synthesis due to its environmental sustainability and capacity for enantiomerically pure product formation. Among various microbial processes, the fermentation of starch-based substrates stands out due to the abundance and renewability of raw materials like corn and potatoes.Hydrolysis of...
Production of Antibiotics
Penicillin, one of the earliest and most widely used antibiotics, is produced industrially by the filamentous fungus Penicillium chrysogenum. Large stirred-tank bioreactors ranging from tens to hundreds of thousands of liters maintain tightly controlled temperature, pH, and dissolved oxygen conditions to support fungal metabolism and maximize antibiotic yield. Penicillin is a secondary metabolite, synthesized primarily during the stationary growth phase, which requires a carefully managed...

