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通过代谢工程在工业Saccharomyces cerevisiae中增强血红素生产

Hyun-Jae Lee^1,2, Tae-Gi Lee^1,2, Changhee Cho³

¹Department of Food Science and Biotechnology, Chung-Ang University, Anseong, Gyeonggi, Republic of Korea.

NPJ science of food

|November 21, 2025

概括

研究人员设计了一个酵母细胞工厂,用于增强血生产. 基因改造在批发发酵中提高了海姆产量的1.7倍,显示了工业应用的潜力.

科学领域:

生物技术是生物技术.
合成生物学合成生物学
微生物工程微生物工程

背景情况:

血红素是一种必不可少的分子,具有多种应用.
开发高效的微生物血生产对于工业供应至关重要.
细菌菌提供了一个强大的平台,用于代谢工程.

研究的目的:

设计一个高效的Saccharomyces cerevisiae细胞工厂,以增加血红素的生产.
优化发酵条件以最大限度地提高血红素产量.
评估工程酵母对工业供应的潜力.

主要方法:

选择并优化了工业Saccharomyces cerevisiae菌株 (KCCM 12638) 以获得高自然血红度.
使用CRISPR/Cas9基因组编辑来过度表达关键的血生物合成基因 (HEM2,HEM3,HEM12,HEM13).
禁用HMX1基因以防止血红蛋白降解和改善积累.

主要成果:

工程菌株 (ΔHMX1_H2/3/12/13) 在批发发酵中达到9mg/L的血标位,比野生类型增加1.7倍.
在葡萄糖限制下进行的料批发发酵产生了67mg/L的血红素.
通过有针对性的基因工程来显著提高血红素生产.

结论:

工业酵母菌株的工程化是一种可行的策略,可以显著提高生产.
开发的酵母细胞工厂显示了可扩展的血供应的前景.
潜在的应用范围涵盖食品,制药和生物能源行业.

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