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来自植物病原菌Ganoderma orbiforme在生物反应器中培养的多糖的生物活性和毒性

Danial 'Aizat Norhisham¹, Jo Cheah¹, Nur Ardiyana Rejab^2,3

¹Functional Omics and Bioprocess Development Laboratory, Institute of Biological Sciences, Faculty of Science, Universiti Malaya, 50603, Kuala Lumpur, Malaysia.

Scientific reports

|November 21, 2025

概括

这项研究揭示了G. orbiforme隐藏的生物技术潜力,为潜在的生物勘探提取安全有效的抗菌和抗氧化多糖体 (EPS和IPS). 这些化合物显示出对健康和环境福祉的可持续应用的希望.

科学领域:

生物技术是生物技术.
微生物学微生物学
植物病理学植物病理学

背景情况:

植物病原菌G. orbiforme导致油棕树的基底干腐烂,使其在生物技术应用中变得阴暗.
多糖,包括细胞外 (EPS) 和细胞内 (IPS) 形式,已知具有多样化的生物活动.

研究的目的:

在空气L形生物反应器中研究G. orbiforme的生长概况.
描述来自G. orbiforme的EPS和IPS的抗菌和抗氧化特性.
为了评估这些多糖的毒性,使用斑马鱼胚胎毒性 (ZFET) 试验.

主要方法:

在空气L形生物反应器中培养G. orbiforme,以确定最佳的生物质和多糖产量.
抗菌检测 (最小抑制度) 针对特定的病原体.
抗氧化剂测定 (DPPH和FRAP) 量化激素清除和降解功率.
斑马鱼胚胎毒性 (ZFET) 试验,以评估安全性和非毒性.

主要成果:

观察到的最大生物质产量为2.53g/L,EPS和IPS的产量分别为0.15g/L和0.06g/L.
EPS表现出更广泛的抗菌活性,特别是在Streptomyces griseus和Staphylococcus epidermidis (MIC 5 mg/mL) 上.
无论是EPS还是IPS,都表现出显著的抗氧化活性 (DPPH IC50:EPS的15.59毫克/毫升,IPS的26.85毫克/毫升) 和降低功率 (FRAP:~74毫米Fe (II) /g).
ZFET测定表明毒性低,EPS的LC50值为1.88毫克/毫升,IPS的LC50值为1.56毫克/毫升,斑马鱼胚胎中没有发育异常.

结论:

G. orbiforme具有重要的生物技术潜力,产生具有显著抗菌和抗氧化特性的生物活性EPS和IPS.
提取的多糖是安全的使用,这是ZFET试验中低毒性的证据.
这项研究支持对植物病原体G. orbiforme进行生物勘探,以实现与可持续发展目标15 (生活在陆地) 和可持续发展目标3 (健康和福祉) 相一致的可持续应用.

关键词:

草皮虫 (Ganoderma orbiforme) 是一种有形的植物.生物活性生物活性液体种植的种植方式棕油是一种油.聚糖类是一种多糖类.斑马鱼胚胎毒性对斑马鱼胚胎有毒性

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