在不同的生物样本采集和储存条件下评估HV稳定性:对分子诊断的影响
Jackson Alves da Silva Queiroz1,2,3,4, Mônica Barcellos Arruda5, Tárcio Peixoto Roca1,2,4,6
1Laboratório de Virologia Molecular, Fundação Oswaldo Cruz Rondônia - FIOCRUZ/RO, Fiocruz Rondônia, Rua da Beira, 7176, Porto Velho, 76812-245, Brazil.
Virology journal
|November 22, 2025
概括
在各种储存温度下,型肝炎病毒 (HDV) RNA 在样本中可检测长达96小时. 这种稳定性对于在偏远地区扩大分子诊断和具有挑战性的储存条件至关重要.
科学领域:
- 病毒学 病毒学
- 分子诊断学 分子诊断
- 公共卫生 公共卫生
背景情况:
- 巴西亚马逊地区的肝炎三角洲病毒 (HDV) 感染率很高.
- 样品运输和储存方面的挑战,特别是在偏远地区,阻碍了对HV的分子诊断工作.
研究的目的:
- 为了评估在不同温度和储存条件下肝炎三角病毒 (HDV) RNA的稳定性.
- 提供支持在资源有限的环境中扩大HVD分子诊断能力的数据.
主要方法:
- 分析了慢性HDV患者的全血,血清和血样本.
- 样品经过各种温度和储存时间的测试.
- 使用定量逆转录聚合酶链反应 (RT-qPCR) 进行了HDV-RNA检测.
主要成果:
- 高密度VRNA在各种温度范围内表现出稳定性,包括高温条件,对Ct值的影响最小.
- 在室温,4°C,-30°C和-80°C保存的样本显示HDVRNA可检测长达96小时.
- 从干血斑点 (DBS) 样本中也可以检测到,尽管与冷保存样本相比,敏感性降低了.
- 当样本存储在4°C至42°C之间时,高密度病毒RNA可检测长达72小时.
结论:
- 在各种温度和储存条件下,型肝炎病毒RNA非常稳定.
- 这些发现支持在具有挑战性的物流环境中对HV的分子诊断的可行性.
- 高密度病毒稳定性数据对于改善偏远和服务不足的人群的诊断可访问性至关重要.
相关概念视频
01:25Hepatitis
80
Hepatitis is an inflammatory condition of the liver most commonly caused by hepatotropic viruses (A–E), though non-infectious causes such as alcohol and drugs also exist.Hepatitis AHepatitis A virus (HAV) is a non-enveloped RNA virus of the Picornaviridae family. It is primarily transmitted via the fecal-oral route, typically through ingestion of contaminated food or water. After ingestion, HAV enters the bloodstream through the oropharynx or intestinal epithelium and reaches the liver.
80
01:28Viral Hepatitis I: Introduction
21
Viral hepatitis is an inflammatory condition of the liver caused by infection with hepatotropic viruses, most commonly hepatitis A, B, C, D, and E. Despite variations in structure and transmission, all viruses mentioned infect hepatocytes and provoke immune responses that can hinder liver function. Additionally, some non-hepatotropic viruses can also lead to hepatic inflammation.Hepatitis A VirusHepatitis A virus (HAV) is transmitted through the fecal–oral route, typically by ingestion...
21