相关实验视频

Updated: Jan 6, 2026

Detection of Alternative Splicing During Epithelial-Mesenchymal Transition

Detection of Alternative Splicing During Epithelial-Mesenchymal Transition

Published on: October 9, 2014

与检查点免疫疗法反应相关的替代拼接的单细胞映射

Jieyi Xiong^1,2, Orian Bricard^1,2, Ingrid Arijs^1,2

¹Laboratory for Translational Genetics, Department of Human Genetics, KU Leuven, 3000 Leuven, Belgium.

Nucleic acids research

|November 22, 2025

概括

单细胞中的替代RNA拼接 (AS) 揭示了瘤异质性和潜在的瘤抗原. 这种拼接对于癌细胞响应免疫检查点阻塞 (ICB) 治疗至关重要.

科学领域:

在瘤学瘤学.
分子生物学分子生物学
免疫学免疫学免疫学

背景情况:

替代RNA拼接 (AS) 涉及到瘤生物学和瘤抗原的产生.
了解瘤微环境中的AS异质性对于开发向疗法至关重要.

研究的目的:

开发一种在单细胞5'-RNA测序数据中检测AS的方法.
描述AS异质性和动态变化在瘤中的单个细胞类型.
确定AS的潜在媒介及其在癌症和免疫反应中的作用.

主要方法:

单细胞5'-RNA测序数据分析.
细胞类型特定的拼接事件的识别.
拼接事件与拼接调节器表达的相关性.
拼接调节器的功能验证,如ESRP1.
优先考虑拼接事件作为潜在的瘤抗原.

主要成果:

许多AS事件被确定为特定于癌细胞,树皮细胞或不同乳腺癌亚型 (TNBC与ER+).
拼接调节器ESRP1被确定并验证为乳腺癌中AS的驱动者,该驱动器对免疫检查点阻塞 (ICB) 作出反应.
来自AS事件的聚合瘤抗原负载与免疫活性相关,并预测ICB治疗患者的T细胞扩张和改善生存率.

结论:

建立了一个用于分析单细胞数据中的AS的新框架.
AS在ICB治疗的反应中发挥着重要作用.
来自AS的瘤抗原代表了癌症免疫治疗的有希望的途径.

更多相关视频

Using the E1A Minigene Tool to Study mRNA Splicing Changes

Using the E1A Minigene Tool to Study mRNA Splicing Changes

Published on: April 22, 2021

Merging Absolute and Relative Quantitative PCR Data to Quantify STAT3 Splice Variant Transcripts

Merging Absolute and Relative Quantitative PCR Data to Quantify STAT3 Splice Variant Transcripts

Published on: October 9, 2016

相关概念视频

Alternative RNA Splicing

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...