微RNA-181a,181b,181c和181d在正牙牙移动中的作用:一个前性队列研究
Monisha Mohan1, Venkatachalam Deepa Parvathi2, Sridevi Padmanabhan1
1Department of Orthodontics and Dentofacial Orthopedics, Sri Ramachandra Dental College & Hospital, Sri Ramachandra Institute of Higher Education and Research, Porur, Chennai, India.
概括
这项研究发现,在正牙牙移动期间,牙沟液中的microRNA (miRNA) -181亚型会增加. 这些miRNAs显示出作为跟踪牙移动阶段的生物标志物的潜力.
科学领域:
- 生物分子标记物 生物分子标记物
- ортодонтика研究 ортодонтика研究
- RNA生物学的RNA生物学
背景情况:
- 非编码RNA因其在正统牙牙运动中的作用而受到关注.
- 这项研究重点关注微RNA (miRNA)-181a,181b,181c和181d作为潜在的生物标志物.
研究的目的:
- 评估miRNA-181a,181b,181c和181d在牙裂液 (GCF) 中的表达.
- 评估这些miRNAs作为潜在的生物标志物在正统牙牙运动的不同阶段.
主要方法:
- 在四个时间点从接受固定器械治疗的患者中采集了GCF样本:基线,24小时,7-14天和强力应用后14-21天.
- 总RNA被分离,量化并转化为cDNA.
- 使用定量实时聚合酶链反应 (qPCR) 来测量四种miRNA-181亚型的表达.
主要成果:
- 在基线时,GCF中检测到了所有四种miRNA-181亚型.
- 分别在24小时和7-14天后,miRNA-181b和miRNA-181a的表达显著增加.
- 在14-21天后,所有四种miRNA亚型的表达显著增加,与基线相比.
结论:
- 在整个正统牙科牙移动过程中,miRNA-181亚型的表达逐渐增加.
- 在任何给定的时间点上都没有观察到亚型之间的显著差异.
- 这些发现表明miRNA-181亚型是监测正统牙牙运动的潜在生物标志物.
相关概念视频
01:22MicroRNAs
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...
01:22MicroRNAs
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...