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Updated: Jan 10, 2026

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界面工程环境活动-稳定性交易-在L-氨酸阿尔多酶中进行高产量的L-threo-MTPS

Yingqi Ruan¹, Wenchi Zhang², Rongzhen Zhang¹

¹School of Biotechnology, Jiangnan University, Wuxi 214122, P. R. China.

Journal of agricultural and food chemistry

|November 22, 2025

概括

接口工程增强了L-氨酸阿尔多酶 (LTA) 的稳定性和活性,改善了β-基-α-氨基酸 (β-HAA) 的合成. 这一突破推动了有价值的氨基酸衍生物的工业生产.

科学领域:

生物催化和酶工程生物催化和酶工程
蛋白质工程是指蛋白质工程.
工业生物技术工业生物技术

背景情况:

对于合成β-基-α-氨基酸 (β-HAA) 来说,L-氨酸阿尔多酶 (LTA) 是至关重要的.
现有的LTA变种受到有限的热稳定性和活动稳定性权衡的影响,阻碍了工业应用.
针对子单元接口的非保存残留物提供了一个潜在的酶改进策略.

研究的目的:

通过界面工程来增强 *Faecalimicrobium dakarense* LTA 的热稳定性和催化活性.
研究子单元接口突变对酶性能和稳定性的影响.
使用工程LTA优化农业化学前体的生产.

主要方法:

使用局部定向突变发生,在子单元接口引入特定的氨基酸替代物 (L17F/V129R/L206W).
在不同的条件下评估了酶活性,热稳定性 (T50^60,Tm) 和产品产量.
用分子动力学模拟来阐明增强酶性能的结构基础.

主要成果:

M3变种的特定活性增加了1.23倍,并且显著改善了热稳定性 (T50^60的+20.5°C,Tm的+3.1°C).
在1L系统中,M3实现了农业化学前体L-threo-4-methylsulfonyl-phenylserine的2.49倍的产量.
缩放到10L显示了高转换率 (95.7%),二聚体过量 (84.9%de) 和时空收益率 (10.5g·L−1·h−1).

结论:

接口工程有效地解开了LTA中的活动稳定性权衡,从而产生了优越的生物催化剂.
该M3变体显示了β-HAA和相关化合物的工业合成的巨大潜力.
这一策略为改善生物技术应用中的多重酶提供了一种可通用的方法.

关键词:

在L-threonine的阿尔多酶.接口工程接口工程接口工程合理的设计理性的设计.稳定性 - 活动权衡权衡.这是β-基-α-氨基酸.

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