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细胞对流体剪切应力的机械记忆和由原子力显微镜揭示的基质刚度

Ziyang Meng¹, Lianqing Liu², Mi Li²

¹State Key Laboratory of Robotics and Intelligent Systems, Shenyang Institute of Automation, Chinese Academy of Sciences, Shenyang, 110016, China; University of Chinese Academy of Sciences, Beijing, 100049, China.

Biochemical and biophysical research communications

|November 22, 2025

概括

癌细胞从它们的微环境中发展出机械记忆,改变细胞机制. 这项研究揭示了液体剪切应力和基板性如何影响这种记忆,影响癌症的进展.

科学领域:

癌症生物学癌症生物学
机械生物学机械生物学
生物物理学的生物物理.

背景情况:

瘤微环境中的机械力量影响癌细胞的行为和转移.
了解癌细胞如何感知和记住机械信号至关重要,但有限.
原子力显微镜 (AFM) 是测量单细胞机械性能的一个关键技术.

研究的目的:

为了研究癌细胞的机械记忆,以应对流体剪切应力和基质刚度.
开发一个模拟瘤微环境的体外模型,用于机械研究.
探索机械刺激对细胞力学的协同效应.

主要方法:

集成原子力显微镜 (AFM),可逆结合微流体和可调节的刚性水凝.
在实验室中构建一个瘤微环境模型,控制流体剪切应力和细胞外矩阵 (ECM) 刚度.
在不同微环境条件下对个体癌细胞进行现场机械测量.

主要成果:

增加的液体剪切应力和/或ECM刚性导致癌细胞变硬.
在随后的环境中,癌细胞保留了改变的机制作为"机械记忆".
确定了流体剪切应力和基质刚性的协同效应,对印记细胞力学产生影响.

结论:

这项研究提供了一种基于AFM的新型实验方法来研究癌细胞机械记忆.
了解机械记忆对于推进癌症机械生物学和治疗策略至关重要.
这项研究揭示了微环境中的物理线索如何塑造癌细胞行为.

关键词:

原子力显微镜的原子力显微镜.细胞机械记忆细胞机械记忆流体剪切应力是流体剪切应力.水凝是一种水凝.可逆结合的微流体学基板的刚性基板的刚性

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