相关实验视频

Updated: Jan 10, 2026

Fabrication and Characterization of Layer-By-Layer Janus Base Nano-Matrix to Promote Cartilage Regeneration

Fabrication and Characterization of Layer-By-Layer Janus Base Nano-Matrix to Promote Cartilage Regeneration

Published on: July 6, 2022

一个潜伏的TGF-β结合性支架改善了新软骨发育

Tianbai Wang¹, Yifan Peng², Bor-Lin Huang²

¹Division of Materials Science & Engineering, Boston University, Boston, MA, USA.

Acta biomaterialia

|November 22, 2025

概括

提供潜伏转化生长因子-β (LTGF-β) 的生物灵感支架促进受控的新软骨发育. 这种方法可以改善软骨的组成和机械特性,模仿本地组织,避免高剂量的TGF-β的不良影响.

科学领域:

生物材料科学生物材料科学
组织工程是组织工程.
再生医学是一种再生医学.

背景情况:

传统的转化生长因子-β (TGF-β) 补充剂用于新软骨发育使用超生理剂量,导致不良的软骨特征,如增生和异质性.
原生软骨通过细胞激活的潜伏复合体 (LTGF-β) 调节TGF-β,确保适度的生理剂量.
目前的方法很难复制天然软骨再生中发现的TGF-β的受控,局部输送.

研究的目的:

开发和评估一种生物灵感的策略,使用LTGF-β结合的支架来控制新软骨工程中的TGF-β输送.
为了比较LTGF-β支架的疗效与传统的活性TGF-β介质补充剂 (MS) 在不同的剂量.
评估LTGF-β支架对新软骨构成,结构,机械特性和细胞表型的影响.

主要方法:

创建了LTGF-β结合的支架,并在糖结构中播种了牛体红细胞.
构造物与补充活性TGF-β在生理学 (0.3 ng/mL) 和超生理学 (10 ng/mL) 剂量或没有TGF-β的构造物进行了比较.
评估包括机械特性,sGAG含量,细胞形态,原分布,基因表达 (COL-I,COL-X) 和组织异质性.

主要成果:

LTGF-β支架实现了与原生相匹配的机械特性和sGAG含量,细胞形态和原分布类似于质软骨.
与无TGF-β和MS-10组相比,这些支架显著降低了COL-I和COL-X表达,这表明了最佳的原体表型.
LTGF-β支架最大限度地减少了机械和生化异质性,超过了MS-0.3和MS-10组的表现.

结论:

结合LTGF-β的支架提供了一个生物灵感的平台,用于在生理水平上局部,细胞介导的TGF-β激活.
这种方法成功地改善了新软骨组成,结构,材料特性和细胞表型,减轻了传统TGF-β传递的问题.
LTGF-β支架策略为改善软骨再生结果提供了一个有希望的解决方案.

关键词:

生物材料脚手架是生物材料脚手架.软骨组织工程软骨组织工程提供增长因素的交付.潜伏转化生长因子β的潜伏转化生长因子β.

更多相关视频

Fabrication of Decellularized Cartilage-derived Matrix Scaffolds

Fabrication of Decellularized Cartilage-derived Matrix Scaffolds

Published on: January 7, 2019

Author Spotlight: Enhancing Bone Regeneration with Vascularized Artificial Cartilage Integration

Author Spotlight: Enhancing Bone Regeneration with Vascularized Artificial Cartilage Integration

Published on: July 14, 2023

相关概念视频

Growth of Cartilage and Bone Tissue

Growth of Cartilage and Bone Tissue

Chondrocytes form a temporary cartilaginous model by dividing and secreting a thick gel-like extracellular matrix. Once the chondrocytes undergo programmed cell death, osteoblasts enter the site of the cartilaginous model. The process of replacing the temporary cartilaginous model with bone in an ordered manner is called endochondral ossification. In endochondral ossification, not all of the cartilage is replaced by bone tissue. Some cartilage that performs a protective and supportive function...

TGF - β Signaling Pathway

TGF - β Signaling Pathway

The TGF-β signaling pathway regulates cell growth, differentiation, adhesion, motility, and development. TGF-β ligands that induce TGF-β signaling are synthesized in their latent form. Several proteases or cell surface receptors such as integrins act upon the latent form, releasing the active ligand. There are three types of mammalian TGF-βs: (TGF-β1, TGF-β2, and TGF-β3) that bind as homodimers or heterodimers to TGF-β receptors. The TGF-β receptors...