人类肠道的prophage景观确定了一个prophage介导的fucosylation机制,缓解大肠炎
Hongyan Gao1,2,3, Yating Wang4, Yulin Zhao1,2
1Center for Infection Biology, School of Basic Medical Sciences, Tsinghua University, Beijing, China.
Nature communications
|November 22, 2025
概括
人体肠道病毒组含有许多菌体,这些菌体与年龄,地理位置和疾病有关. 一个特定的prophage基因有助于产生2-fucosyllactose (2′-FL),它可以通过改善肠道屏障功能来减少结肠炎.
科学领域:
- 微生物学 微生物学
- 免疫学 免疫学 免疫学
- 胃肠病学 胃肠病学
背景情况:
- 人体肠道病毒组的功能,尤其是 prokaryotic 基因组内的 prophages,仍然在很大程度上是未知的.
- 在人类肠道微生物群中,食原体很丰富,但它们的多样性和作用尚未得到充分研究.
研究的目的:
- 为了识别和描述人类肠道微生物群中的prophage序列.
- 为了研究prophage丰富和多样性与宿主因素如年龄,健康和地理因素的关联.
- 探索体编码基因在健康和疾病,特别是炎症性肠道疾病中的功能作用.
主要方法:
- 对289,232个人类肠道元基因组基因组进行生物信息分析,以识别益生菌序列.
- 对8503个肠道基因组样本进行统计分析,以与宿主和环境因素相关联 lysogeny 比率.
- 在体外酶试验和体内小鼠模型 (德克斯硫酸盐诱导的大肠炎) 验证futC基因及其产物2'-FL.的功能.
主要成果:
- 确定了254,273个prophage序列,显著扩大已知的肠道prophage多样性.
- 素比率因年龄,健康状况和地理位置而异,具有疾病特异性变异的prophage基因患病率.
- 负责2'-FL生物合成的prophage编码的futC基因在炎症性肠病患者中不太常见;通过增强粘膜屏障完整性和调节免疫反应,2'-FL给药减少了小鼠的结肠炎.
结论:
- 体溶解发生与人类宿主特征和环境因素有关.
- 体编码的futC基因及其产物2'-FL在调节肠道炎症方面发挥作用.
- 体编码的基因可以具有免疫调节功能,为肠道疾病 (如结肠炎) 提供潜在的治疗途径.
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