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MicrobiomePrime:用于微生物源追踪的初始对选择工具,该工具在动物肠道和便废物微生物组的综合收集中得到验证

Tanja Zlender¹, Lucija Brezočnik², Vili Podgorelec²

¹Department for Microbiological Research, National Laboratory of Health, Environment and Food, Prvomajska 1, Maribor, 2000, Slovenia; Department of Microbiology, Faculty of Medicine, University of Maribor, Taborska 8, 2000, Maribor, Slovenia; Biotechnical Faculty, University of Ljubljana, Jamnikarjeva 101, 1000, Ljubljana, Slovenia.

|November 23, 2025

概括

MicrobiomePrime自动化了微生物源跟踪 (MST) 的原料设计,提高了水质分析. 这种生物信息学工具有助于有效地识别便污染源,保护公共健康.

科学领域:

环境微生物学环境微生物学
生物信息学是一种生物信息学.
水质管理水质管理.

背景情况:

微生物源追踪 (MST) 对于识别水中的便污染至关重要.
目前基于PCR的MST方法取决于精确的原始设计用于标记器放大.
开发新的MST测试需要专门的生物信息学专业知识.

研究的目的:

推出MicrobiomePrime,这是一个自动化的生物信息学管道,用于设计MST原料对.
为了实现有效和准确的原料设计微生物源跟踪从amplicon测序数据.
简化新型微生物源追踪试验的开发.

主要方法:

开发了MicrobiomePrime,这是一个基于k-mer的自动化生物信息管道.
利用来自各种动物和人类便样本的16S rRNA基因扩增序列数据.
设计和验证了用于微生物源追踪试验的新型原料对.

主要成果:

MicrobiomePrime成功设计了针对各种便来源和废物的新型MST测定.
在体外验证显示了51个设计试验的高性能.
18个测试实现了100%的特殊性,证明了管道的实用性.

结论:

MicrobiomePrime自动化了微生物源追踪的原料设计,减少了生物信息学障碍.
管道促进了水质管理的敏感和特定测试的开发.
该工具通过改善便污染源的识别来支持公共卫生保护.

关键词:

动物来源动物来源便污染便污染卡梅尔 (K-MER) 是一个词.标记器标记器标记器微生物源追踪微生物源追踪

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