相关实验视频

Updated: May 8, 2026

ELIME Enzyme Linked Immuno Magnetic Electrochemical Method for Mycotoxin Detection

ELIME Enzyme Linked Immuno Magnetic Electrochemical Method for Mycotoxin Detection

Published on: October 23, 2009

同时检测四环素和阿弗拉托克辛M1使用构造由分子印记和阿普坦尔功能化的Janus粒子

Weilong Zhao¹, Baixin Ye¹, Feng Liu²

¹School of Chemistry and Chemical Engineering, North Minzu University, Yinchuan 750021, China; Ningxia Key Laboratory of Solar Chemical Conversion Technology, North Minzu University, Yinchuan 750021,China; Key Laboratory for Chemical Engineering and Technology, State Ethnic Affairs Commission, North Minzu University, Yinchuan 750021, China.

Journal of chromatography. A

|November 23, 2025

概括

一种新的Janus材料可以同时检测牛奶中的阿弗拉托辛M1 (AFM1) 和四环素 (TC). 这一进步通过对这些常见的牛奶污染物进行准确和高效的分析,提高了食品安全.

科学领域:

食品科学与技术食品科学与技术
分析化学分析化学
生物技术是生物技术.

背景情况:

牛奶被阿弗拉托克辛M1 (AFM1) 和四环素 (TC) 污染造成了严重的食品安全风险.
同时检测这些污染物的方法对于乳制品的有效监测和控制至关重要.

研究的目的:

设计和合成一个双功能Janus材料,用于同时识别和检测AFM1和TC.
开发一种集成的固相提取 (SPE) 和液态染色学-并联质谱法 (LC-MS/MS) 方法,用于分析牛奶中的这些污染物.

主要方法:

开发 Janus-MIP-Apt 材料,用于 TC 的分子印记聚合物 (MIP) 位点和用于 AFM1.1 的吸收体 (Apt).
使用Janus材料作为固相提取 (SPE) 吸附剂用于样品制备.
使用液体染色学-并联质谱法 (LC-MS/MS) 进行提取的AFM1和TC的定量分析.

主要成果:

雅努斯-MIP-Apt材料表现出高吸附能力和对TC和AFM1.1的选择性.
集成的SPE-LC-MS/MS方法显示了广泛的线性检测范围.
取得了令人满意的康复 (86.7105.8%) 和低检测极限 (0.1μg/L用于TC,0.2μg/L用于AFM1).

结论:

开发的Janus-MIP-Apt材料是一种有效的吸附剂,可以同时从牛奶中检测AFM1和TC的SPE.
综合SPE-LC-MS/MS方法为牛奶中这些污染物的同时确定提供了可靠和敏感的方法,有助于提高食品安全.

关键词:

非洲毒素M1 非洲毒素M1 亚普特美尔 (Aptamer) 是一种药物.亚努斯粒子亚努斯粒子这是LC-MS/MS.固体相提取方法固体相提取方法环素四环素的使用方法

更多相关视频

Visual Detection of Multiple Nucleic Acids in a Capillary Array

Visual Detection of Multiple Nucleic Acids in a Capillary Array

Published on: November 15, 2017

Aptamer-Based Target Detection Facilitated by a 3-Stage G-Quadruplex Isothermal Exponential Amplification Reaction

Aptamer-Based Target Detection Facilitated by a 3-Stage G-Quadruplex Isothermal Exponential Amplification Reaction

Published on: October 6, 2022

相关概念视频

Mass Analyzers: Overview

Mass Analyzers: Overview

The mass analyzer is a crucial component of the mass spectrometer. In the ionization chamber, the vaporized sample is bombarded with a high-energy electron beam to generate a radical cation and further fragment into neutral molecules, radicals, and cations. A series of negatively charged accelerator plates accelerate the cations into the mass analyzer. The mass analyzer separates ions according to their mass-to-charge (m/z) ratios and then directs them to the detector. The common types of mass...

Mass Analyzers: Common Types

Mass Analyzers: Common Types

The quadrupole mass analyzer consists of four cylindrical metal rods arranged in a diamond carrying a DC voltage and a radio-frequency AC voltage. The motion of ions through the quadrupole depends on the field strength, causing only ions of a certain m/z to resonate successfully and strike the detector at a given field strength. Though the transmission rate for these analyzers is high, the exact elemental composition of the sample is not determined because of low resolution; however, they are...

Gas Chromatography: Types of Detectors-II

Gas Chromatography: Types of Detectors-II

In gas chromatography, different detectors are employed to meet specific analytical needs. These detectors are often categorized based on their detection mechanisms and the types of compounds they are best suited to analyze. Thermal Conductivity Detectors (TCD), Flame Ionization Detectors (FID), and Electron Capture Detectors (ECD) represent common categories, each with unique operating principles and applications. However, beyond these, several other detectors are designed for more specialized...