通过CRISPR-Cas调解对抗性基因的向,以对抗ESKAPE病原体感染:一篇综述
Mohd Faiz Khan1, Mohd Javed1, Jashanpreet Kaur1
1Department of Biotechnology, National Institute of Pharmaceutical Education and Research, SAS Nagar, Punjab, India.
International journal of biological macromolecules
|November 23, 2025
概括
克里斯普尔-卡斯系统提供了一种新的方法来打击抗菌素耐药性,通过精确地准危险细菌中的耐药性基因. 这项技术在提高耐药性病原体的敏感性方面显示出前途,可能会彻底改变感染治疗.
科学领域:
- 微生物学 微生物学
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
背景情况:
- 抗菌素耐药性 (AMR) 是一个日益增长的全球健康威胁,特别是来自ESKAPE病原体.
- 传统药物对抗多药耐药细菌菌株的有效性越来越低.
- 克里斯普尔-卡斯系统提供精确的基因编辑能力,与打击AMR有关.
研究的目的:
- 审查ESKAPE病原体中抗微生物耐药性基因的机制.
- 突出CRISPR-Cas技术在准这些抗性基因方面的成就.
- 讨论在细菌病原体中的CRISPR-Cas组件的传递系统.
主要方法:
- 审查关于CRISPR-Cas在抗菌素耐药性的应用现有文献.
- 对各种CRISPR-Cas系统 (例如,CRISPR-Cas9,Cas3,dCas9,迷你CRISPR) 的分析.
- 探索新的策略,如攻击-克里塔和克里斯普尔干扰.
- 讨论传递方法,包括工程细菌菌体,纳米颗粒和细菌结合.
主要成果:
- 克里斯普尔-卡斯系统有效地准关键的耐药基因 (例如,tetM,erMB,VanA,blaNDM).
- 克里斯普尔-卡斯技术展示了对抗生素耐药细菌敏感化的潜力.
- 工程化菌体和其他新型传递系统显示出对体内应用的前景.
结论:
- 克里斯普尔-卡斯技术代表了对抗多药耐药病原体的强大战略.
- 使用CRISPR-Cas针对抗性基因可以逆转细菌中的抗性表型.
- 进一步开发传递系统对于基于CRISPR的AMR疗法的临床转化至关重要.
相关概念视频
01:28CRISPR/Cas9 Genome Editing
1.6K
The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
1.6K
01:59CRISPR
57.4K
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
57.4K
02:53CRISPR and crRNAs
18.7K
Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
18.7K
01:23The Antiviral System of Bacteria and Archaea: CRISPR
601
CRISPR stands for Clustered Regularly Interspaced Short Palindromic Repeats is a adaptive immune system found in bacteria and archaea that protects against viral infections. This system enables prokaryotic cells to identify, remember, and neutralize foreign genetic elements, primarily bacteriophages, by storing fragments of the invader’s DNA as a genetic memory.The CRISPR immune response begins during an initial infection. Cas (CRISPR-associated) proteins play a central role in this...
601
02:31Homologous Recombination
62.5K
The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
62.5K
00:49What is Genetic Engineering?
79.6K
Overview
79.6K