[从白血病还原镜残留物中分离出来的双阴性T细胞的功能性特征]
Zhiqiang Xiang1,2, Yue Wu3, Kaiyu Huang3
1Department of Pharmacy, School of Pharmaceutical Sciences, Zhejiang Chinese Medical University, Hangzhou 310053, China. xiangzq@126.com.
概括
白血病还原过器残留物为双阴性T细胞 (DNT) 提供了可行的来源,其生物特性与全血细胞相似. 这些DNT细胞在冷保存后保持其特征,这表明细胞治疗制造的潜力.
科学领域:
- 免疫学 免疫学 免疫学
- 细胞生物学 细胞生物学
- 生物技术是生物技术.
背景情况:
- 双阴性T细胞 (DNT) 是一种具有治疗潜力的特异性T细胞群.
- 目前用于DNT细胞隔离的方法可能会限制治疗应用的可扩展性.
- 白血病减少过器是血液加工的副产品,可能含有有价值的细胞材料.
研究的目的:
- 为了评估DNT细胞的生物特性,从白血病还原镜残留物中分离出来.
- 为了比较来自过残留的DNT细胞与来自全血的DNT细胞的特征.
- 评估冷保存对来自过残留的DNT细胞的活力和功能的影响.
主要方法:
- 流血还原过器被加工,以隔离DNT细胞的起始材料.
- 通过抗体介导吸附和密度梯度离心分离DNT细胞.
- 在新鲜和冷保存的细胞上进行了体外培养,免疫类型,活力,扩张和细胞毒性测试.
主要成果:
- 从过器中恢复白细胞的比例为41.9%.
- 过残留的DNT细胞在培养后表现出与全血DNT细胞相似的扩张,免疫表型,分化和细胞毒性.
- 虽然最初的纯度和活力较低,但从残留物中冷保存的DNT细胞保持了生物活性,一些功能在解后得到增强.
结论:
- 白血病还原过器残留物是DNT细胞分离的有希望的来源,产生具有与全血相比较的生物功效的细胞.
- 从过残留物中获得的DNT细胞在冷保存和复苏后保留了它们的生物特征.
- 这些发现支持使用白血病还原过器残留物作为制造现成DNT细胞治疗药物的有价值的起始材料.
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