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相关概念视频

Proteomics01:33

Proteomics

9.2K
A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...
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Ribosome Profiling02:24

Ribosome Profiling

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Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
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相关实验视频

Updated: Jan 10, 2026

Cell Type-specific Gene Expression Profiling in the Mouse Liver
10:06

Cell Type-specific Gene Expression Profiling in the Mouse Liver

Published on: September 17, 2019

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在体内特定于细胞类型的细胞内特异性分泌物形造型.

Jie Liu1, Toren Finkel1, Shihui Liu2

  • 1Aging Institute of University of Pittsburgh and University of Pittsburgh Medical Center, Pittsburgh, PA, United States; Division of Cardiology, Department of Medicine, University of Pittsburgh School of Medicine, Pittsburgh, PA, United States.

Methods in cell biology
|November 23, 2025
PubMed
概括

研究人员开发了一种新的方法,使用近距离生物化来识别特定组织的分泌蛋白质. 这项技术应用于新的"Secretome Mouse"模型,成功地分析了内皮和肌肉蛋白质,推进了蛋白质组研究.

关键词:
生物ID BioID 是一个生物ID.生物素是生物素中的一种.内皮细胞内皮细胞的内皮细胞.肌肉 肌肉 肌肉 肌肉亲近生物化 (proximity biotinylation) 是一种生物化方法.一个秘密,一个秘密.

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"Cell Surface Capture" Workflow for Label-Free Quantification of the Cell Surface Proteome
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"Cell Surface Capture" Workflow for Label-Free Quantification of the Cell Surface Proteome

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Cell-Type Specific Protein Purification and Identification from Complex Tissues Using a Mutant Methionine tRNA Synthetase Mouse Line
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Cell-Type Specific Protein Purification and Identification from Complex Tissues Using a Mutant Methionine tRNA Synthetase Mouse Line

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相关实验视频

Last Updated: Jan 10, 2026

Cell Type-specific Gene Expression Profiling in the Mouse Liver
10:06

Cell Type-specific Gene Expression Profiling in the Mouse Liver

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8.2K
"Cell Surface Capture" Workflow for Label-Free Quantification of the Cell Surface Proteome
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"Cell Surface Capture" Workflow for Label-Free Quantification of the Cell Surface Proteome

Published on: March 24, 2023

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Cell-Type Specific Protein Purification and Identification from Complex Tissues Using a Mutant Methionine tRNA Synthetase Mouse Line
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Cell-Type Specific Protein Purification and Identification from Complex Tissues Using a Mutant Methionine tRNA Synthetase Mouse Line

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科学领域:

  • 蛋白质组学是指蛋白质组学.
  • 生物化学 生物化学
  • 分子生物学分子生物学

背景情况:

  • 血清蛋白质组的复杂性源于不同的细胞和组织起源.
  • 确定组织特异性对循环蛋白质组的贡献仍然具有挑战性.

研究的目的:

  • 开发和验证一种分析组织特异性分泌蛋白质的方法.
  • 为了建立一个小鼠模型,以在体内阐明体内分泌的秘密.

主要方法:

  • 利用内网膜穿越蛋白质的近距离生物化,用于体外分泌物形.
  • 开发了一种"秘密基因小鼠"模型,用于体内特定组织的秘密基因分析.

主要成果:

  • 在体内成功确定了内皮细胞特异性蛋白质分泌物.
  • 通过使用模型进行运动影响的肌肉衍生血清蛋白的特征.
  • 证明了Secretome鼠标模型的广泛适用性.

结论:

  • 近距离生物化对于秘密基因组造型有效.
  • 机密基因鼠标模型使得体内特定组织的机密基因分析成为可能.
  • 这种方法有助于更好地了解循环蛋白及其组织起源.