在DNA损伤反应中,AtMCM10通过液-液相分离促进SDSA介导的分子间同源重组修复
Xinjie Zhao1, Tianren Zhang2, Dan Jin2
1Hunan Province Key Laboratory of Crop Sterile Germplasm Resource Innovation and Application, College of Life Sciences, Hunan Normal University, Changsha, China. sinjo2013@163.com.
Nature communications
|November 23, 2025
概括
迷你染色体维护10 (MCM10) 对于基因组稳定至关重要. 这项研究表明AtMCM10在DNA修复中的功能,特别是在植物中通过DNA回火和液体-液体相分离 (LLPS) 促进同源重组 (HR).
科学领域:
- 分子生物学分子生物学
- 遗传学 遗传学 是一个
- 植物科学 植物科学
背景情况:
- 迷你染色体维护10 (MCM10) 对于基因组稳定至关重要,参与DNA复制和修复.
- MCM10在DNA修复途径,特别是同源重组 (HR) 中的精确作用需要进一步阐明.
研究的目的:
- 为了研究Arabidopsis thaliana MCM10 (AtMCM10) 在DNA修复机制中的功能.
- 阐明AtMCM10参与DNA双链断裂 (DSB) 修复的分子机制.
主要方法:
- 在基因毒性和盐度压力下评估Arabidopsis thaliana突变物中的DNA损伤水平.
- 分析AtMCM10在同源重组 (HR) 途径中的作用,包括合成依赖链化 (SDSA).
- 采用细胞学和生物化学分析来研究AtMCM10的DNA回火活性,DSB位点的局部化以及液态-液态相分离 (LLPS) 特性.
主要成果:
- 在压力条件下,AtMCM10功能的丧失会加剧DNA损伤.
- AtMCM10在ATM的下游运行,对于SDSA介导的分子间HR至关重要.
- AtMCM10表现出DNA回火活性,局部化到DSB位点,并通过单链DNA (ssDNA) 促进LLPS进行LLPS.
结论:
- 在植物体细胞中,AtMCM10作为一个ssDNA回火蛋白,对SDSA介导的分子间HR修复至关重要.
- AtMCM10利用LLPS促进DNA修复,为基因组稳定性维护提供了新的见解.
- 这项研究强调了AtMCM10在DNA复制和修复中的双重作用,强调了它对基因组完整性的重要性.
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